GEO help: Mouse over screen elements for information.
Status
Public on Aug 01, 2007
Title
CUFSL Listeria 6K oligonuclotide array v1.0
Technology type
spotted oligonucleotide
Distribution
custom-commercial
Organisms
Listeria monocytogenes ; Listeria innocua
Manufacturer
Cornell University Microarray Core Facility, T2 002B Vet Research Tower, Cornell University, Ithaca, NY 14853 USA
Manufacture protocol
2,857 70mer oligonucleotides were designed by and purchased from Operon Biotechnologies, Inc. (Huntsville, AL, USA) to represent 2,857 ORFs from Listeria monocytogenes EGD. 70mer oligonucleotides were designed for 61 Listeria innocua ORFs (based on L. innocua Clip 11262) and five Saccharomyces cerevisiae ORFs (to serve as negative controls) using ArrayOligoSelector (http://arrayoligosel.sourceforge.net ). Oligonucleotides were resuspended in 1X SSC + 0.0025% Sarkosyl to a final concentration of 25uM. All oligonucleotides and controls were spotted in duplicate on Corning UltraGAPS slides (Corning, NY, USA) using a custom built XYZ microarrayer (based on the original design of Pat Brown from Stanford University). Oligonucleotides were UV-crosslinked at 300mJ for 1 minute. Printing quality was checked using Cy3-labeled random sequence nonamers.
Support
glass
Coating
aminosilane
Contributor(s)
Raengpradub S , Wiedmann M , Boor KJ
Submission date
Mar 23, 2007
Last update date
Aug 01, 2007
Contact name
Sarita Raengpradub
E-mail(s)
sr266@cornell.edu
Organization name
Cornell University
Department
Food Science
Lab
Food Safety Lab
Street address
412 Stocking Hall
City
Ithaca
State/province
NY
ZIP/Postal code
14853
Country
USA
Samples (54)
GSM180671 , GSM180672 , GSM181533 , GSM181534 , GSM181535 , GSM181536
GSM182055 ,
GSM182058 ,
GSM182086 ,
GSM286188 ,
GSM286189 ,
GSM286190 ,
GSM286191 ,
GSM286192 ,
GSM286193 ,
GSM286194 ,
GSM286195 ,
GSM286196 ,
GSM286197 ,
GSM286198 ,
GSM286199 ,
GSM286200 ,
GSM286201 ,
GSM286202 ,
GSM286203 ,
GSM286612 ,
GSM286614 ,
GSM286615 ,
GSM286616 ,
GSM286617 ,
GSM286618 ,
GSM286642 ,
GSM286643 ,
GSM423310 ,
GSM561855 ,
GSM561894 ,
GSM562207 ,
GSM562217 ,
GSM562218 ,
GSM562219 ,
GSM562220 ,
GSM562221 ,
GSM562222 ,
GSM562223 ,
GSM599934 ,
GSM599935 ,
GSM599936 ,
GSM599937 ,
GSM599938 ,
GSM659585 ,
GSM1171883 ,
GSM1171884 ,
GSM1171885 ,
GSM1171886
Series (10)
GSE7465
Listeria monocytogenes Cold Regulon in Log- and Stationary-Phase cells
GSE7492
Listeria monocytogenes and Listeria innocua SigB Regulons as defined by salt-stressed and early stationary-phase cells
GSE7514
CtsR Regulon in Listeria monocytogenes
GSE7517
HrcA Regulon in Listeria monocytogenes
GSE11347
Characterization of the SigB-PrfA regulatory network in Listeria monocytogenes
GSE16887
Identification of sigB inhibitors in L. monocytogenes
GSE22672
Listeria monocytogenes Grown at Refrigeration Temperature Shows Reduced Acid Survival and an Altered Transcriptional Response to Acid Shock
GSE24339
SigH, SigL, and SigC regulon in Listeria monocytogenes 10403S
GSE26818
A small RNA controls expression of the chitinase ChiA in Listeria monocytogenes
GSE48187
Listeria monocytogenes exposure to chlorine dioxide
Data table header descriptions
ID
Block
Block number (16 blocks total)
Row
Row number within a block (20 rows per block)
Column
Column number within a block (20 columns per block)
ORF
Entrez Gene Link
Chip_ID
ID assigned by Operon or spot type assigned by CUFSL
SEQUENCE
Oligo sequence
SPOT_ID
Data table
ID
Block
Row
Column
ORF
Chip_ID
SEQUENCE
SPOT_ID
lmo0205
1
1
1
lmo0205
LM00002168
TTTGCCGGGTTTTGCTAATGCGAAAATAACAGGAGCAAAGTATTTCAATCAATCGGTGACTGATTACCGA
lmo0207
1
1
3
lmo0207
LM00002170
CACTAAAATCTCTTCGGAAGATATGAACAAAATCCCTGGTATGTCTTCAAATGGTGATACTTCTAAAGGG
lmo0209
1
1
5
lmo0209
LM00000872
TGGAATATCGCTTTTCGCGAAACATGGTCACAACTAACTTTTGGACTAGCATCGATTGTAAGCAAACGTG
lmo0211
1
1
7
lmo0211
LM00000068
TAAAGCTGGCGGTGTACTTCAACAAATTATCCATGATGTAAAAGTTTCAGCAACACCAGAAAAATTACCA
lmo0213
1
1
9
lmo0213
LM00000873
CCGGCATAATGTCGGTTTTATGGTAGTTGACGAGCTGAGTTTTCGTCATCAAACCCCTTGGAAGAAATCA
lmo0215
1
1
11
lmo0215
LM00000875
TTTACCGAGTACCCTTTCAAAATATGGTGGGGGATGTAGGGTTTTATATTTTCCAACAAGTATATCCGAT
lmo0229
1
1
13
lmo0229
LM00000882
ATTTACAATGGAACGAGGGTATATTGTTGAAAGCAAACGTGGTGGTGGTGGATATATTCGGATTATTAAA
lmo0231
1
1
15
lmo0231
LM00000073
GCCAAAAAATGAATGAATTGGTACTATTCTCGCAACCAGCTTTTTTGAGAAGAGAAGCAGGACGAGATAT
lmo0233
1
1
17
lmo0233
LM00000884
ATCACGCTCAGCTTTCAATCATACAGGAGAGCCTTCAAAAGCGACTCCAATTACTCAAATAGCAAGTGAA
lmo0235
1
1
19
lmo0235
LM00000885
GTTGGCGAACCAATTTTCATTCATGCCTTGCGTCCTTTTTTAGCGGATAATCGTTGTTCCAAAGTGATTG
lmo0237
1
2
1
lmo0237
LM00000886
TATAATGCATTTGGCTGGGAGCCACCAATTTTTGGGCATATGACACTTATTGTGAATGAGAGCAGACGTA
lmo0239
1
2
3
lmo0239
LM00000887
TATCGTGGTTATGATGTGAAATTCGTGTCTAATTTTACGGATGTGGATGATAAATTGATTCGTGCGGCTA
lmo0253
1
2
5
lmo0253
LM00002178
AATAAGCGGATTCAAATGGATCGTGAATTAGCGTGTGATGAAGCTGTTTTAGCTACATTGTCGAAAAGTA
lmo0255
1
2
7
lmo0255
LM00002180
TTCCCAATACTCTGCATATAAAGAGAGCAAAGGAGCTTCACTAGAAACGAAATTTACTGATGAAGCGCTA
lmo0257
1
2
9
lmo0257
LM00002181
AGGCTCTGACGGTATTTACCTTGTAATCCATAGTGGAAGTCGGGTGCTTGGTAAAGAAATCGCGGAATAT
lmo0259
1
2
11
lmo0259
LM00000083
CACAAGCAGAAGCACGTATTTTAATGCTTGCAGCTCAAAACATTTTGAACCCTAAAGATGGTAAACCAGT
lmo0261
1
2
13
lmo0261
LM00000084
GCGCAAACATATCGAACAAATGAAAGAAGCAATTGCTGACGGCGTAGATTTAATGGGTTACACAAGCTGG
lmo0263
1
2
15
lmo0263
LM00002183
CAAGTAATTCAGGAGGCAATACCACACTTCCAACAGCTGGTGACGAAAATACCATGCTTCCGATTTTTAT
lmo0277
1
2
17
lmo0277
LM00000902
ACCACTATGAATTTATCATGAAAAGTTTACATAATGGCAAACACGTTTTAGCTGAAAAAGCGATAACTGT
lmo0279
1
2
19
lmo0279
LM00000089
GAAGGAATGTTATCAAAAGGATTTACGATTGGTAATGCCAACGTAGAAAGCCCGAAATCTATCCAAACAG
Total number of rows: 3200 Table truncated, full table size 313 Kbytes .
Supplementary file
Size
Download
File type/resource
GPL5029.txt.gz
108.7 Kb
(ftp) (http)
TXT