Meyerowitz laboratory at the California Institute of Technology
Manufacture protocol
The oligonucleotides of the Arabidopsis Genome Oligo Set (Operon) were provided in 384-well plates. The dried DNA was resuspended in 15 ul of printing buffer (150 mM Na-phosphate pH 8.5) per well, resulting in an estimated DNA concentration of 40 uM. DNA was spotted onto poly-lysine coated glass slides with a MicroGrid II spotter (Biorobotics) using Microspot 10K quill pins (Biorobotics). After printing, the slides were processed and blocked following standard protocols.
Support
glass
Coating
polysine
Description
This microarray is based on the Arabidopsis Genome Oligo Set Version 1.0 (Operon, Alameda, CA). This set consists of 26,090 oligonucleotides that correspond to 22,361 annotated Arabidopsis genes (The Arabidopsis Information Resource (TAIR) genome annotation version 6).
Transcription repressor HANABA TARANU (HAN) controls flower development via integrating multiple hormone actions, floral organ specification and GATA3 family auto-regulation
Data table header descriptions
ID
CLONE ID
Gene_Symbol
Gene symbol of gene represented by an array element (according to TAIR)
Gene_Description
Description of gene or gene product represented by an array element
ORF
GenBank GENE
SPOT_ID
spot identifier
Data table
ID
CLONE ID
Gene_Symbol
Gene_Description
ORF
SPOT_ID
A000001_01
At1g58360_1
AAP1
Organic Solute CotransportersAmino acid permease family amino acid permease I (AAP1), identical to amino acid permease I GI:22641 from (Arabidopsis thaliana) F19C14.3, F19C14_3, NAT2, NEUTRAL AMINO ACID TRANSPORTER 2
transducin family protein / WD-40 repeat family protein, contains 4 WD-40 repeats (PF00400); similar to neural cell adhesion molecule 2, large isoform precursor gb:M76710 from Xenopus laevis, and beta transducin from S. cerevisiae gb:Q05946. ESTs gb:N65081 gb:Z30910, gb:Z34190, gb:Z34611, gb:R30101, gb:H36304, and gb:N65606 come from F20D22.9, F20D22_9
Protein synthesis factorsEF1Bgamma - elongation factor 1BgammaAteEF1B-G1 elongation factor 1B-gamma, putative / eEF-1B gamma, putative, Similar to elongation factor 1-gamma (gb:EF1G_XENLA). ESTs gb:T20564,gb:T45940,gb:T04527 come from this gene F21M12.3, F21M12_3
NPR1/NIM1-interacting protein 1 (NIMIN-1), identical to NIMIN-1 protein (Arabidopsis thaliana) gi:12057154:emb:CAC19844; identical to cDNA NIMIN-1 protein (nimin-1 gene)GI:12057153 T6A9.23
similar to Ulp1 protease family protein [Arabidopsis thaliana] (TAIR:At1g10570.2); similar to Ulp1 protease family protein [Arabidopsis thaliana] (TAIR:At1g10570.1); similar to OSJNBb0021A09.14 [Oryza sativa (japonica cultivar-group)] (GB:NP_916015.1); contains InterPro domain SUMO/Sentrin/Ubl1 specific protease (InterPro:IPR003653) T13D8.11, T13D8_11