GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Platform GPL5849 Query DataSets for GPL5849
Status Public on Sep 27, 2007
Title Host and Pathogen Microarray
Technology type spotted DNA/cDNA
Distribution non-commercial
Organisms Homo sapiens; Human gammaherpesvirus 8
Manufacturer Custom spotted at UCL
Manufacture protocol The purified DNA probes in spotting buffer were deposited onto aminopropyl silane-coated Nexterion Slide A (Schott, UK) using the Qarray2 arrayer (Genetix, UK) with a 12-pin print head of Genetix aQu 75um split pins (Genetix, UK). Spotted arrays were air-dried for 12+ hours, followed by heating at 80 °C in dry oven for 3 h to immobilise the DNA. Unbound surfaces on the arrays were inactivated by immersing in 60 ml blocking solution: 5× SSC, 0.5% (w/v) bovine serum albumin (BSA, Sigma, UK), 0.5% (w/v) non-fat powdered milk (Tesco, UK) at 50 °C for 45 min, followed by washing 2 times in deionised water. Then the arrays were incubated in deionised water at 95 °C for 2 min to denature the DNA. Denatured arrays were washed and blocked again (with fresh solution) at 50 °C for 30 min. Finally the arrays were washed 2 times in deionised water, immersed in isopropanol (Sigma, UK) for 30 seconds and immediately dried by spinning at 200 g, 2 min. Processed arrays were stored in a dry environment for use within 24 hours.
Catalog number NA
Support glass
Coating aminosilane
Contributor(s) Li C, Tsao E, Kellam P, Choi K
Submission date Sep 13, 2007
Last update date Sep 27, 2007
Contact name Paul Kellam
Phone 00442076799559
Organization name UCL
Department Infection
Lab Virus Genomics & Bioinformatics
Street address 46 Cleveland Street
City London
State/province London
ZIP/Postal code W1T 4JF
Country United Kingdom
Samples (15) GSM230311, GSM230312, GSM230313, GSM230314, GSM230315, GSM230316 
Series (1)
GSE9096 XBP-1 transduction of PEL

Data table header descriptions
Name Gene Name
GB_ACC GenBank Accession number

Data table
HP150 40s ribosomal protein s18 40s ribosomal protein s18
HP2198 40s ribosomal protein s3A 40s ribosomal protein s3A
HP1425 60s Acidic Ribosomal Protein P 60s Acidic Ribosomal Protein P
HP4242 60s ribosomal protein L30 60s ribosomal protein L30
HP4364 8D6 antigen AA463987
HP5634 8D6 antigen AA434501
HP1170 alu subfamily alu subfamily
HP4875 annexin A6 AA975225
HP3986 apoptosis antagonizing transcr AI439571
HP6024 apoptosis antagonizing transcr W72325
HP266 ATP-binding cassette sub-fami AA255836
HP2329 a-tubulin K00558
HP2331 a-tubulin K00558
HP3083 B aggressive lymphoma gene AA431459
HP3353 b-actin X00351
HP3355 b-actin X00351
HP3865 Basic probe X56932
HP3867 Basic probe X56932
HP1174 CAMP-regulated phosphoprotein CAMP-regulated phosphoprotein
HP1924 caspase recruitment domain fam W68684

Total number of rows: 771

Table truncated, full table size 29 Kbytes.

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary file Size Download File type/resource
GPL5849_full_array.txt.gz 39.4 Kb (ftp)(http) TXT

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap