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Platform GPL5958 Query DataSets for GPL5958
Status Public on Jan 17, 2008
Title Lactobacillus johnsonii NC533 microarray project
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Lactobacillus johnsonii NCC 533
Manufacturer Eurogentec - parc industriel d'Ivoz-Ramet 21 - 4400 - Ivoz-Ramet - Belgium
Manufacture protocol Primer synthesis of 1876 pairs of primers in 96 well Multiplex format
Each Forward primer carries a Tag sequenceand a total number of 9 different tags have been designed for covering all Forward primers. All reverse primers contains a unique tag
Following a 96 well plate map, a specific arrangement of these 9 tags provides a way to eliminate one major source of DNA contamination during the secondary amplification by locating one tagged PCR product away from the adjacent one that will be amplified with identical tags. Basically, tags 1 to 9 are arranged in a row starting from position A1 to A9, and this is repeated in the next positions and second lane of the plate, i.e. well plates B1 and B2 will contain primers tagged with 4 and 5, respectively
Primary PCR amplification: The reverse primers were mixed with the corresponding Forward primers and diluted each to 5 mM final concentration to prepare a 10-fold concentrated solution of specific primers
A master mix is prepared with EuroTAQ incubation buffer, EuroTAQ thermostable polymerase (EGT), 0.2 mM each dNTPs (EGT), 1.8 mM MgCL2, LA-1 genomic DNA (5 ng), and dispensed by 90 µl to the 96 well PCR plates.
PCR amplification were performed in Applied Biosystem thermocycler GeneAmp PCR system 9700 with 1 min at 94°C for initial denaturation followed by 30 cycles (94°C, 1 min; 50°C, 30 sec; 72°C, 1 min), and finally a final step at 72°C for 5 min.
An aliquot of each PCR mix (5 µl) was resuspended in EthBr loading buffer and analyzed by agarose gel electrophoresis (1% in TAE buffer).
The size of amplicons were determined with the help of the molecular weight DNA ladder Smart Ladder (EGT).
Secondary PCR amplification : The unique Reverse Tag primer was mixed with the corresponding Forward primers 1 to 9 in order to prepare a template 96 well plate that was used for transferring the tag primer couples at the good locations in all 96 well PCR plates (10 µl, 5 mM final concentration)
We obtained 98.6% of the aminated secondary PCR products, which covers 1876 ORFs
PCR purification was performed by using the Millipore Multiscreen 96 well Filter Plates.
Based on differential binding of DNA molecules on silica matrix, this system is adapted for centrifugation with a throughput of 17 plate per centrifuge.
A binding buffer (Guanidine-HCl7 M; MES 200 mM pH = 5.6) is first added to the PCR plates.
Following up and down pipetting, the samples are transferred to the purification plate for binding onto the glass fibers during centrifugation.
One step centrifugation washing is done with Ethanol 80 %. Amplicons are recovered in H20 buffered at pH 8 (120 µl).
Water have been removed by evaporation and DNA samples were resuspended to a final volume of 30 µl 3x SSC printing buffer (average concentration 100-200 ng/µl).
An aliquot of each first lane (A1 to A12 well) from 96 well plates (1 µl) was resuspended in EthBr loading buffer and analysed by agarose gel electrophoresis (1% in TAE buffer).
For arraying the aminated PCR products, the samples were transferred from 96 well plates to V-bottom 384 well plates (Genetix #7020).
Additional controls (printing buffer, C1, C2, C3 and luciferase cDNA from Renilla) were added in the last 384 well plate in order to place these controls in each subgrid of the array (16 subgrids in total).
We used the Virtek ChipWriterPro, equipped with 16 SMP3 quill pins (Array It, Telechem) for arraying the library in close duplicates with 200 µm dot spacing onto CMT-GAPS II Corning glass slides.
Each subgrid contains 16 columns and 16 rows.
In order to provide an area for normalising the laser intensities for working at an optimal dynamic range and the scans for normalisation on intensities recovered from spike control RNA (LuxA control RNA available at Promega), we added an extra lane located outside of the main array frame that contains serial two-fold dilutions of sonicated LA-1 genomic DNA, Luciferase cDNA and CAT cDNA.
Following printing, the slides were dried overnight at room temperature in the gridder chamber and processed according to the Anhydride succinic acid protocol recommended by Corning.
Printed microarray slides were incubated in MilliQ water for 2 min at 95°C for DNA denaturation.
For checking the quantity and quality of DNA spotted onto the glass slides, we performed a Cy5-dCTP Terminal Transferase labelling (Roche) of bound DNA molecules onto the ready-to-use LA-1 microarray glass slides (one slide per 40 slides-batch).
For this purpose, we directly added to the printed glass slide area, 20 µl of dCTP labelling reactional mix according to the manufacturer’s recommendations.
A coverslip was applied onto the array and the glass slide was incubated for 15 min at 37°C.
The glass slide was then washed 5 min in 0.2x SSC, 0.1% SDS and 5 min in 0.2x SSC and finally spin-dried.
Microarray glass slides were scanned with Genepix 4000 scanner (Axon instruments, Inc.) and arrays analysed with Genepix 4.0 software.
 
 
Submission date Oct 04, 2007
Last update date Jan 17, 2008
Contact name Emmanuel Denou
E-mail(s) emmanuel.denou@rdls.nestle.com
Phone + 41 21 785 9315
Fax + 41 21 785 8544
Organization name Nestlé Research Center
Department Nutrition & Health
Lab Food & Health Microbiology
Street address vers chez les blancs
City Lausanne
State/province vaud
ZIP/Postal code PO Box 44-CH-1000 Lausanne 26
Country Switzerland
 
Samples (18) GSM234674, GSM234675, GSM234676, GSM234677, GSM234678, GSM234679 
Series (1)
GSE9236 In vitro expression profiles of Lactobacillus johnsonii NCC533 in MRS

Data table header descriptions
ID
INTERNAL_ID LJ ID
Description Gene Description (Pridmore et al., PNAS, 2004)
TIGR id Gene function attributed by TIGR C = Cell envelope; D = Cellular processes; F = DNA metabolism; G = Energy metabolism; H = Fatty acid and phospholipid metabolism; I = Hypothetical proteins; J = Mobile and extrachromosomal element functions; K = No Data; N = Purines, pyrimidines, nucleosides, and nucleotides; O = Regulatory functions; P = Signal transduction; Q = Transcription; S = Unclassified; T = Unknown function; U = Amino acid biosynthesis; V = Biosynthesis of cofactors, prosthetic groups, and carriers; R = Transport and binding proteins ; Z =no TIGR main role
SEQUENCE DNA sequence of spotted amplicon
Amp L length of the amplicon sequence

Data table
ID INTERNAL_ID Description TIGR id SEQUENCE Amp L
1 Ljo_0001 Chromosomal replication initiator protein dnaA Z agctacagcacggttgcctataatgcttggtttaaaaatactaaaccaatttcctttaataaaaagacaaaagaaatgataatcgctgttgaatcacccgttgcaaagggatactgggaaaagaacttagcttctcaactcattcaagaagcatatgcttatgccgacatggaaattcaacctaaatttgaagttgcaggtaaagaaggtcctgaacgtttagttacaccacaaccacgaattaaaactaatcaagaaatattagaaaatcgtagagatgaatttgctcaagaccttcaactaaatagtaagtatactttcgatacttttgttcaaggtgagggaaacaagctggcagcgggtgcagccttagcagtcgctgataatccgggaagtttttacaatcctttgtttatttttggtggagttggtttaggtaaaacccacttaatgcaagccattggacaccaaatgttggctgaaaaaccacatgcaaaagtggtttatattcagagtgaaactttcgtaaatgattttattaactcaataaaaaacaaaactcaggctgaatttcgtaataagtatcggaactgtgatctcctattagtagacgatattcaattcttttctaaaaaagagggaatccaggaggaattcttccatacttttgaaaccctttataatgatcaaaaacagattgttatgactagtgatcgtctaccaaccgaaattc 733
2 Ljo_0002 DNA polymerase III, beta chain (EC 2.7.7.7) FS ctatgcgtgcaatttcttcacgtgctactattccaatattaagtggtataaaacttacccttacagatgaaatgcttactttaaccggtagtgatactgacatttctattgaaattcaaatcccagtaaacgatgatttaattgttcaatctacgggatcgatcgttttaccagcacgatttttcagtgaaattgtaaaaaaattaccaggtaaagatttttcatttgaagtaaaagaaagttttcaaacgaaaattgtttctgaaaataccgaattcatgattaacggtttagatgcaaacaattatccacacttaccagaaatttctactgatgcatcattcaaaatttctggtaaaacatttagagaaattattaacgaaactgtttttgcagtcgctactcaagaaagtcgtccaactttaactggtgttaactttatcttcaacaattcatcaattaaagcagttgctaccgacagtcaccgactatctcaacgtcaaatttctctagaaaatggtcctcaaacaagtactgacttaattattcctggaaagagcttagtagaattatcccgaattatcggagaaagtgatcctgaaatcacagtaaatcctggtgaaaaccaagttttatttgaagttggaaacattgcattctattcacgtttacttgatggacaatatccagacactgatc 699
3 Ljo_0003 DNA replication and repair protein recF F tggtccaaacgcccaaggaaaaactaatttacttgaggcgatttattttttggccctaactcgatctcatcgaacaaatagcgataaagaattaattcgttttggaagtaaatttgcgggcttacaaggaaaagttcataaaagtcaattagaagtagagttaaaattacgtttaacaccaaacggaaaaaaagcttgggtaaatagactcgagcaaaagaagctttccgcttatgtaggacaaatgaatgcaattttgttttctcccgaagacctagccctggttaaaggagctccatcaattagaagacgttttatggatttagagtttgggcaaattaattcagaatatctatattttttaagtcagtatcgtcaggtactgcaacaaagaaacaattacttaaagcaattgagcataaaaaaggcaaatgatttagtttttttagatgtcttgtcagatcagcttgcaggaattgcagcggagataatttctagaagaatcaaatatattaaaaaacttaattcatatgctcaaagtgctcatagtgagattagtggtcaagctgaaaaattacagattttttatcgtccatcagttaaggaaattaccccagatgatgatgtagagaccatttatcaaaaagttattactagttataaaaaaaatcgtcctaatgaaattcgaaaggggactactttgagtggac 710
4 Ljo_0004 DNA gyrase subunit B (EC 5.99.1.3) F gcagaagataagagcaaggcagcattaaataaagctaaagattttgaaaagcgtgctgatacttataacgcaagtcaaattcaagttctaggcggacttgaagcagttagaaagcgcccaggtatgtacattggctcaactagtagccaaggcttacaccaccttgtttgggaaattattgataatagtattgacgaacgattagcaggatttgcgaccaagattgaaattactgttaatgaagatggcagtgttacagtccaagatgatggacgtggtattccggttgatattcaagctaaaacaggtcgtccagccctagaaactgtatttactgttttgcatgctggtggtaaattcggcggtggcggatataaagtatctggtggtcttcacggtgttggtgcttctgtagtaaacgccctctcaactaaacttgaggttactgttatgagggatggaaagaaatatgcaatttcttttgatcatggccgtgtagtaggagaaatgaagcaagtgggaactgtacctcttgaagaacatggaacaatcgttcgtttctatcctgatccagatatttttacagaaactacaactttcgacgataaaattttaaagaaccgaattcgtgaacttgctttcttaaacaagggcttaaaattaacctttaccgataagcgtaaagaaagtgctgaaactgatgtttaccactttgaaggtggaatcaaagaatacgttgcct 742
5 Ljo_0005 DNA gyrase subunit A (EC 5.99.1.3) F gtattactggtgtacgggatgaatcagaccagactggtatgagaattaccattgatattcgccgtgatgcaagtgctagtgtggttttgaataatttatttaaagaaacccaaatgcaagcaaacttcggaatgaatatggtggccattgttaatggtgcaccgcatttcctaactttgaagcaaatgcttgagtactaccttcaccaccaagaagatgttattactcgtagaactaagtttgagttaaagaaggctgaagctagagcacatattttagctggattaagaattgcccttgatcatatcgatgagatcattaagattattcgcggttcacaaaatagcgatattgctaaggcacaattaattcaaaactttggcttagacgatcgtcaatctcaagctattttagatatgagattggttcgtttaacaggcttagagcgcgataaaattgaagctgaatatcaagatttgcaagcaaaaatagcagattacaaagatatcttagctaaaccagaacgaattgatgagattatttacaacgagttacttgatattcaaaagcgttttggtgatgatcgtcgtactaagattgccgaaggtgaagcagtttcaattgaagatgaagatttaattgaacaaaaagatgttcttttaactctaactcataatggctacattaagcgtatgccagcggatgaattcaaggttcaaaaccgcggtgg 730
6 Ljo_0006 SSU ribosomal protein S6P M gttacttacatcatcaagcctgatattgacgaagattcaaagaaagcattgatcgatcgtttcgataaagttgttactgacaacggtgctgaagaacttgaatctaaagattggggtaagagacgtttcgcatacgaaattgaaaaataccgtgaaggtacttatcacattatgactttcgttgcagaaaactctgaaccagttgatgaatttggtcgtctttctcgtattgataacc 238
7 Ljo_0007 Single-strand DNA binding protein S ggaatctcagttgctacgtttactctagctgttgaccgtcaatataccaatagtcaaggtgagcgtggtgctgatttcatcagctgtgtaatttggcgtaaagcagcagaaaattttgctaactttacttcaaagggttcattagttggtattcaaggtcggattcaaactagaacgtacgatgataaagatggtaagagagtatacgtgactgaagtcattgttgataatttctctttactagaatcacgtcgtgatcgtgaaaatcgtcagactaatggtggtaattttgctccgcaaggaggaaatgctccaagta 319
8 Ljo_0008 SSU ribosomal protein S18P M gctcaacaaagaagaggtggccgtcgtcgtcgcaaggttgactttattgcagctaaccacattgactacatcgactacaaagatgttgatttgttgaaacgttttatctctgaaagaggtaagatcttaccacgtcgtgtcactggcactagcgcaaagaatcaacgtaagttgactgttgctattaagagagctcgtgttatgggcttattgccattcgtcgcagaaga 230
9 Ljo_0009 Magnesium and cobalt transport protein corA W ccgaccttcacgaacgtccccactttgatcatgattatattactaacagtgatttgttggtttacgatgttccagtctggccaacagctgatgcagatcattttacaactttaccaattaagtttttaatggtcgatcatacgctttttactgttcattctcctgatacaacttatatgattgaagaatttaggcaacgaccagatgagcatattcatagtgaaaaggaattgatttttgctattctttttgctgttacaaaatactttcaaagggcacttagtcaactaaacagtcaacgactagttttagataataacttaagtgagcgaattcataataaagacttacaagaattatcacaagttgaaaaaagtttggtttacttatctagctcaattagaactaatctaatgatgcttgaaagtctaaaaaataaaaaatcaggacttcatatgaatgcttctgaagaggaaatgtgtgacgatattatcatcgaagttcagcaatcttcacaaatgatcaaaatttatagtgaagtgactgaagaaatttctaagacatctaataacattctgaataataacttgaacaataccatgcagtttttgactgtgtggtcact 625
10 Ljo_0010 Phosphoesterase, DHH family protein L ccttagcaattacgctggacaatttaattcatatcttaaacgcattgatgaagatcactttattttattgacgcatatgcatgatttagccaagatggaagaagataaattctctattttggataaagttagaactgaatcaagtcgaaagaatatgccattgaccttgtctattgggattgcgtttggctcagaaagcttaaatgaaattgctgatcaagctcaatccaacttagatttagctttaggacgtggtggagaccaagttgttgtaaagcaatcgggtcacgaagcacatttttatggtggaaaatctaatccaatggagaaacgaactagggtaagagctcggatggtttctcaggccttggtagaattatttaagggcgttgaccatatttttgttcaaggacatcgaaatcctgacttagatgcgattggctctgcaattggaattgtaaaaattgctcgaattcatggtgtaaaagctagtgttgttttagatgttgatcacgttaattatgacgtgggacgtttgattgctaaaatgcaagctgccggtatcgataaagatgtatttatttcgcctaaagatgctttagaagaagcaaccgatgagtccttactagttttaacagatcactctaagtacagtattacttatgatccagaactttacgaccggttgaagaat 694
11 Ljo_0011 LSU ribosomal protein L9P M cgtacctgatggctatgctcaaaatttcttgattaaaaatggatatgctaaagaagctactagttctaacttaaataccttgaaacgtgttcaaaaagctgaaaaagatgcttatgaagcagaaaaggctgctgctgaagatattaagaaaaaacttgaagacgacaaaactattgttaactttaagtctaaggctggtactgattctcgcttatttggttctatttcaagtaagaagattgttgaaggacttgaaaagcaatacggaatcaaggttgataagcgtaaattagagcttccagaaccaattaaatcattagggtatacaaatgtacctgttaaattatttaaaggcgtagaagcagtaattcgcgttcacattacggagcaa 391
12 Ljo_0012 Replicative DNA helicase (EC 3.6.1.-) F gtgaagcagaaaaggcggtcctaggggccgtctttttagatcccgaagcaattattgatgcttcagatgtattacagcctgatgatttttatgaacatgcaaatagaattgtttttcaggcaatgcttaatatctcagatcgtgaggaagtaattgacccagttactttacaagatgagttaaagaaaaataatcaagtagatgatattggaggtattgcctatgttacagaactatcaatggcaactccaacagcagcccatgttacttactacgcaaaaattgtaaagcgaaaagctattcttagaaatctaatttctactagtcaaaggattattcaaaatgcaattgaaggttctgatgatgtaactgatatcttagatgatgcagaaagccaaataatgggggtctcccaggataatgcaagtggtgggttcaaatctattcatgatgtattaaacacggctatggaagaaattaactcgattcctgatgatggaaatatggtaacgggtctaccatctggattttctgaattagataagatgacaactggttttcacgatgatgaattaattatcttggctgctcgtcctggtgttggtaaaactgcctttgctcttaatgtggcacaatttgttggactaaaaactgataaaactgttgctatgttctcacttgaaatgggagctga 694
13 Ljo_0013 Fructokinase (EC 2.7.1.4) G cggcagtattgaagctggtggaactaaattcatcttggcagttcaagatacagaaagtggcaaaattgtagctaagaagagaattccaacaacagatgcaaaagaaactttagcaaaaagtattgaattctttaaagagcacccagtagctgctttgggaattggtacttttggtccaattgacatcaatccaaattctagaacatttggttatatcttagatacacctaagcgtggttggtcaggaaccaatgttaaaggcacttttgaaaaagaactaggtattccagtggtaatgactacagacgtgaatgcttcatgttatggtgagtatattgcacgtggaaaagatgattctaagacttatttctatgtaacaattggaacagggattggtgccggcgcggtacaagcaggtaaatttattggtttaaataatcacccagaaatgggccacatgctagtcactccttaccctggcgacaactatactgggaaatgtccatttcatggaaataaatgtgttgaaggaatggcagctggaccttctctagaaggaagaactggtattccaggtgaaaaattacctagagaccacaaggtatttacttatgtttcatactatgtagctcaattattatttaatgcttacatgacaatgcgtccggatgtaatggttgttggtggatcagtactcaatgatcaagacttggtccaagttcgtggattctttagagaatttaataataattatgtcgcaacgccagacgtcgacg 776
14 Ljo_0014 Lipase I attatgttcgctggcgaggggacctaacacttgatcgagaaccatttaatagtttagatgctggactttttgccgcatttgcttatcttccttttgattctagtgttaaagggcatactctcgaagcagctacaaaacgtttaatacaaaaaaagacattaattcattcagataaagtagaagctcaattaatcaaccttagtgatcgatataaaaatatgcgctttttagactggtctcatctttcccagaaaaagccaccagtacagttcacagccatgacaattgaattagatcctcagactattctagtagcctatcgaggaactgatggatccatggtcggactgaatgaagatgtagatatgagttatcagccagaaatttttggacaaagtgtagcagcagactatttagataaaattgcccagacttatcctgataagcaaatttatactactggtcattcaaaaggtggaaattttgctgcttatgctatctctgctgtttcacctaaaattcaagatcaaataataaaagcatatagttttgatggtcctgggtttatgaaagaaacttatgatcaactagaatttcaaagagctattcccaagatgattacctatgttcccgaaggatcaatttttgggatgatgcttgatcatcccgaacgtgtcatcgtagttaaaagcaagatgaaacatttaatgcagcagcataatccccttcattgggaagttgctcgaaatagcttcgttatggctccttgtctcagcaatg 780
15 Ljo_0015 K ggacaaatgacttcatttccggactggcacgctattgaaggcagagactgggctaagctatttgatgctcaagctggtcctggacacccacgtgtagcccggcctcacctaccagcttataacgaagtagctcctttgatttatggacgcaaattcgaagaatatagccgtaatactgctgcgtttttaggcccacatgctgaattatatccagaactagaaaattcaatgtggactccgcaagtaaaatctaatacctttattgcttttcaaattattacgactgcccttcttggtgcaaagggcattatgcttaatatctttgatatgatgggcaacggaattaatgaagattggggctatggcaaaatgcttgcagatattaaaccatttgcttctgccttatctaaccaccgtctcaatatgaaagaattaagaggtattaaagttctagtagatcaggattctgcttatactgctcatactacttggggcaaagagccagaagaattgcttccccatgaaaaaaactgggctagtcttttagccagttttagctttgcaacaactattttgcctattcaagataattttgaaatcgaagatcaaacaattgcaatcgctggtcaactgctgcgcaatcttacagatagtcaaatcactaaactaatcacacaaaatacagttctacttgatggtgaaagtattcaagttttgcttgatcgagatttaggtaacttactccatattcaatcggct 759
16 Ljo_0016 Amino acid permease W cttggatctttggtggtgttattacactagcagctggtttaacaattgctgaaattggagctcaaatgccatatactggcggattatatgtttacattgagaagttatatggtcggattcttggttttctagccggatggatgcaagttatcgtctatggccctgcaattattgcagccgtaggtggttttaccggtattcttatcgccaatttctttaatctaaatgattcctggcgcattcctctatcaattgggtgtatgctttttatcactattattaattttttagataataaggtaggtgcagcattttcagttattactacaattggaaaaatgattccgattgctgcaattattatttttggtatcttttggggtcaaaataatgccttaggtcagactatttctgacgttaaacaaagtagtggcggatttggagttgctgtgttagctaccttgtttgcttacgacggctggattttaattgcgaacttaggtggtgaaatgaaaaatcctcaaaaagtccttccgcaagcgattatcttaggaattacgattgttttaacgatctacactctaattacagtcggtatcttacgctttattccagccgaccttattga 628
17 Ljo_0017 Acetyltransferase (EC 2.3.1.-) I gtatgggggttggcgaatataaagatggaaaattagctagttacatcatggtcaatgaatttgaaagtagaatctttgctaaaaaggtaaaaatgggtggagttggttatgtagcttcatatccagaaaatcgcggtcaaggtgatattaatcgcttgatgaatgaaattattttagagttgtacaggcagaattatgctatttctaatttggcaccattttctgaaagtttttatcgacgttatggctatgaaaatgctatttatgaaaaaatgtataaattaaatccggcttatttgcgcttttttaagtcagttaaagaaggaaaaattgtccgaggaaaatggaaagattcagacttaaaagaagcagtcataaagttatatcaagataagctgaatcaaaataatcaaagaaatactgttgatcgtgaagactggtggtgggatcgtttagatacctattatcctgggagatttatctgtgtctactttgataaaaataatcagccagctggctatatgttctatcgtattcgggatagggagtttagggtgaaggagctgtactataaaacgcctcaagcag 588
18 Ljo_0018 6-phospho-beta-glucosidase (EC 3.2.1.86) S cctaaaaagtttggagagctagttatgattattggtaaaaagcgacaagaggttatttttaatattaaaagatgtgttaaggagaaaaaatttaacgcaaaagttgaaccagatgatccagttttaagcaaaaaagatcgcctaaaattagtagaaaaattttgggctaatcataatagccccttttctaaagctattaatatacttgcacgcggaatccttaacatcggaacgcctcttttaactttaaatactgaaattgataatcctgaatctcttagtaagctttcttcagctattgtaacctgtaatcactacaatcaatttgattgtctccctctgaagcggctcgccatgaaatatcataagcgattatattttgttatcgaagatactaatttaaaactaccaaactggattggctttttaatgaggaatattgatagcatcccggtaactgctagttttcgctatttaggacgtgagctgccaaaacacttaaataaaatcctttctaagaaaaatcgctggattgtggtatatccagaacaagaattatggcttaattatcgaaaacctcgtcctctgcaaaaaggcgcctattattatgcagctaaaatgaatgttccaattatttcatgctttgtagaaattaaggacactaacaagcaagaaaaactgcatcctgaatttaacaaaacacgttggattcttcatgttctacctactatttatcctgatcccaaactctca 753
19 Ljo_0019 Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25) I gtgcttctggaatggcagggagtgctatttataaattagctagtgaaaacaaagatcttgaagttacaggtattgtcagagataaagaaaaggccgaaagagttcttggtaaagatgctcaccttttaattggcgatgttttaacaatggacgatagcttactagaaaactttgatgtaattgttgatgcctttggtacaactcctgaaaaggcaaaggatcaagtaaagctagcaactaaattatgtggtctagctaagaacaaggatattagagtaatctttatcttaggtgcaggtagtcttagaactggaattgataaccacttggtagtggaagacattaagaaattaactggttcagaaaactggattaatacccctcaagagcaattaaacgaattaaactacttaaaacaagttgaagatgtagattggctgggaatttcaccagcattgacttttgaagcaggcccagaggctgcaggttatatgttaggtaacaatactcttctctttaataacaataatgaatctaaggtaacctctggaacaatggctcgcttgattgtaaatgaaattgctcatccaaagcaccataaa 602
20 Ljo_0020 Transcriptional regulator, Xre family O ccggaacaatagtttcaacgtctttctactctcgagtcgaacaggataatactcgtatttcagccattgatttacttaaaattttaaaagtccatcacatcaatatatctgatttcattgatcaactaaccattaaactaaataatcaaactgatgaagatgaaataatagatacactatttattgcattttttaataacgatatagacactgtcatgcaattaaaaaaatatatatacttacgggcatcacaaactacaaaattatatataaaattaattgaagctcttttaaaacaagataggactatattaaataaacaggatgaagatctcatcagaaaagaactatttaacggtttaaattggtcaattccaacattaaaattatttactaattctatgtttatttatacttctgatgagttatcttttctactaacttctattttaaataaatatcaaacaactaaaaatgaaacagttcaaaagttcattgcaagtatttgtataaattttttagatttatgttataaaagaaaaaactttaccttttggaaaaagggaatatcttttttgtttagtttgcctagtactccagaactgttgatgtataaattgcttggcaaatattatcaagccattttagaaaaagataaaactactatagatcatatcaagaccactcttatgctttc 697

Total number of rows: 1876

Table truncated, full table size 1113 Kbytes.




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