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Platform GPL6694 Query DataSets for GPL6694
Status Public on Apr 09, 2008
Title BOAC CattleOligo 13.2K + 8.7K 70-mer microarray v1
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Bos taurus
Manufacturer CRB GADIE
Manufacture protocol 1-1. The oligos set used for this microarray is a mix of the 8.7k set from Operon and a 13.2K
set designed by the Illinois University and synthetised by Illumina.
1-2. Oligos are arrayed by Illumina in Greiner 384-well flat-bottom plates. Each well contains 3000 pmol of 70-mer oligo.
1-3. Oligos are arrayed by Operon in Genetix 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo.
2. Resuspend oligos in water(20µM).
3. Incubate at room temperature during a night shaking plates to aid resuspension of DNA.
4. Deep freeze and defrost centrifuging plates at 1000 rpm for 16 minutes then 2000 rpm for 3 minutes.
5. Take 5 µl of oligos solution and put it in a new genetix polypropylène v-bottom plates(cat# X7022)
6. Add 5 µL of 1X Printing Buffer to each well and mix.
7. Centrifuge plates at 2000 rpm for 3 minutes.
8. Oligos are ready for spotting.
9. Spotting on glass slides, with 24 pins on cheapwriter of Virtek, with control of humidity (45-50%).
10. Fixation of the oligos slides with UV (600 mJoules).
11. Storage the slides in a vacuum.
12. After the print run is completed, cover oligos plates with seals and deepfreeze them at 20°C in a protected environment.
Support glass
Coating aminosilane
 
Description spotted oligonucleotide
 
Submission date Apr 07, 2008
Last update date Apr 09, 2008
Contact name Sylvain Marthey
E-mail(s) abridge@inra.fr
Organization name INRA
Department Animal Genetics
Lab Animal Genetics and Integrative Biology
Street address Domaine de Vilvert Bat 320
City Jouy-en-Josas
ZIP/Postal code 78352
Country France
 
Samples (50) GSM351712, GSM351713, GSM351714, GSM351715, GSM351716, GSM351717 
Series (5)
GSE14008 Goat mammary gland differentiation during pregnancy
GSE31261 Transcriptional profiling of cumulus cells entoured the oocyte after in vitro or in vivo maturation in bovine (Bos taurus)
GSE31361 Gene expression profiling of in vitro matured bovine oocytes cultivated with or without cumulus cells.

Data table header descriptions
ID
Block
Column
Row
GeneName
Accession
SEQUENCE
GB_ACC GenBank or RefSeq accession number
SPOT_ID control, etc.

Data table
ID Block Column Row GeneName Accession SEQUENCE GB_ACC SPOT_ID
bt00000704 1 1 1 homologue to UP|PM5P_HUMAN (Q15155) Protein pM5 precursor, partial (52%) CK770441 ATCAAGCTCCTGTTGGACTGACCCTCCCCTTAACGTCCCTGTGCATTTGAGGTCCCTGCTGGAAACCGCA CK770441
bt00000701 1 2 1 homologue to GB|AAH05010.1|13477111|BC005010 ZFP36L2 protein {Homo sapiens;} , partial (63%) CK770437 AACAGCAGAAGGGGGGCGGCGGCTCCCAGATCAATTCGACGCGCTACAAAACTGAGCTGTGCCGGCCCTT CK770437
bt00000686 1 3 1 homologue to GB|BAA06123.2|473931|HUMORFA01 Start codon is not identified {Homo sapiens;} , partial (20%) CK770394 GTTATAAAGATCCAGTCTTTGGCAAGGATGCACCAAGCTAGAAAGCGCTATAGAGAACGCCTGCAGTATT CK770394
bt00000683 1 4 1 homologue to UP|CCA1_MOUSE (Q8K1J6) tRNA-nucleotidyltransferase 1, mitochondrial precursor (mitochondrial tRNA nucleotidyl transferase, CCA-adding) (mt tRNA adenylyltransferase) (mt tRNA CCA-pyrophosphorylase) (mt tRNA CCA-diphosphorylase) (mt CCA-a CK770384 TTAAGGCAGTAGATAGTTCAGAACCACTGAAACCCTATCAAGACTTCATCATAGACTCTAGAGAAGCTGA CK770384
bt00000680 1 5 1 similar to UP|Q8CHM0 (Q8CHM0) Axotomy induced glycoprotein 3, partial (51%) CK770381 TGCATCTTGTATTCATGTTTGACATCGACGACCAGATCAAGTTCCGATGCTCTGCAGGGCCGGTACACAG CK770381
bt00000677 1 6 1 weakly similar to UP|Q06810 (Q06810) Ypr075cp (Overproduction induced pheromone resistant yeast 2), partial (6%) CK770374 CTTCAAGAAAAACTGAATGAAATGAGCTATGAACTGAAGTCTGCTCAGGAGTCATCTCAGAAGCAAGATG CK770374
bt00000378 1 7 1 homologue to UP|Q86V09 (Q86V09) Fragile X mental retardation syndrome related protein 2, partial (48%) CK769877 TGAACACCCCCATTCTCCGCCTGTTGGTGCGGGGTGCTCCTTGACCCACCCAGATTTGACAGTTCAGGGG CK769877
bt00000375 1 8 1 UP|KC22_BOVIN (P20427) Casein kinase II, alpha chain (CK II) , complete CK769872 CTAGACCAACCGAAGGGCAGCCCACCACCGTATATCAAACCTCACTTCCGAATGTAAAAGGTTCTCACGC CK769872
bt00000372 1 9 1 UP|ACON_BOVIN (P20004) Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase) (Aconitase) , complete CK769869 TTAGTCCGATTCAGATCTTGAGCAGCTTCATGCAACTATTTATTTTTGATGACAGACGCCCGTCTAAAGT CK769869
bt00000369 1 10 1 homologue to GB|BAA23690.2|2662069|AB007854 Start codon is not identified {Homo sapiens;} , partial (82%) CK769863 TACGGCAGCACCTTTGCCAGTACACGCAGCTGCGGCACGAGACGGACATGTTCAACCAAAGCACAGTTGA CK769863
bt00000354 1 11 1 UP|MSRA_BOVIN (P54149) Peptide methionine sulfoxide reductase (Protein-methionine-S-oxide reductase) (Peptide Met(O) reductase) , complete CK769836 GCATGGTTTCGGCCTGATCACCACGGACATCCGGGAGGGACAAACCTTTTACTATGCGGAAGATTACCAC CK769836
bt00000351 1 12 1 similar to UP|Q47393 (Q47393) ORF1b, partial (12%) CK769830 AGAAAATCAGCAGCATAAATGCCTGTTGTTCAAGAGTGCGATCTCCTCCTGCTACTAAATCATGACCAGC CK769830
bt00000348 1 13 1 0 CK769822 CCTGGGCAACCCCGTGCGTCCGTTCTACATGTGCAAACCTCTTCCCCAGTGCCCTTGTGCACTTTGGACA CK769822
bt00000345 1 14 1 similar to UP|Q76LP5 (Q76LP5) ATPase 6, partial (8%) CK769817 TGACTGTTAAGTGATCCCAAGAGCTTTTACTGCCAGATACTTTTCAGGCTTACCAGTAAAGTGAGTTTAA CK769817
bt00000330 1 15 1 homologue to GB|AAD22973.1|4559298|AF125672 silencing mediator of retinoic acid and thyroid hormone receptor extended isoform {Homo sapiens;} , partial (14%) CK769773 GCAGCTTCTGGCTCTCAGCTGCAAAGGCCGGGAAGAACACAGGCTTCTCTGCCTCCTTCTCGGCAGGAGG CK769773
bt00000327 1 16 1 homologue to GB|AAH21254.2|18204189|BC021254 TMEM24 protein {Homo sapiens;} , partial (91%) CK769767 CCTTCCCCAGAGAGATGGCTTTAGGAGTTTTATTTACAGACTGTGTGATGATGGAGCAAGAGCAGGGTTG CK769767
bt00000324 1 17 1 similar to UP|RT05_MOUSE (Q99N87) Mitochondrial 28S ribosomal protein S5 (MRP-S5), partial (92%) CK769759 TCTCCATGTTGTGGAATTCCGGGAGGAATGTGGCCCTCTGCCCATCGTGGTTGCCTCACCACAGGGGGCC CK769759
bt00000321 1 18 1 UP|NU93_MOUSE (Q8BJ71) Nuclear pore complex protein Nup93 (Nucleoporin Nup93) (93 kDa nucleoporin) (CBP-interacting protein 4), partial (68%) CK769756 CAGAAGTGCTTCTCGCCACCATGAACATCTTGTTCACACAGTTTAAGAGGCTCAAGGGGACAAGTGCATC CK769756
bt00000306 1 19 1 UP|RUV2_HUMAN (Q9Y230) RuvB-like 2 (48-kDa TATA box-binding protein-interacting protein) (48-kDa TBP-interacting protein) (TIP49b) (Repressing pontin 52) (Reptin 52) (51 kDa erythrocyte cytosolic protein) (ECP-51) (TIP60-associated protein 54-b , co CK769732 GACCTGCTGGACTGTTTGCTTATCGTTTCCACCTCCCCCGTCAGCGAGAAAGACACAAAGCAGATCCTTC CK769732
bt00000303 1 20 1 homologue to UP|Q9JKD9 (Q9JKD9) Repressor of GATA (Zinc finger protein TZFP), partial (23%) CK769728 ACGGAGCGTCAGTAACTTCTTTGGCTTCAGCCAAGCTTACAATTAAAAAGTGAAAGACGGGCCGGCGAGC CK769728

Total number of rows: 23232

Table truncated, full table size 3667 Kbytes.




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Supplementary data files not provided
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