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Status
Public on May 08, 2008
Title
Custom LC Sciences Arabidopsis microRNA and SiRNA array
Technology type
in situ oligonucleotide
Distribution
custom-commercial
Organism
Arabidopsis thaliana
Manufacturer
LC Sciences (Houston, TX, USA)
Manufacture protocol
MicroRNA probes were designed by LC Sciences (based on Sanger Institute miRBase repository sequences on August 2, 2005). See http://www.lcsciences.com for additionnal details. Some SiRNA probes were from ASPR (http://asrp.cgrb.oregonstate.edu/ ), another were designed according to the sequence information of NRG1 gene (Zuhua He et al., Science, 2000, Vol.288, pp.2360-2363) LNA-modified RNA probes were synthesized in situ using photogenerated acid coupled with conventional DMT chemistry (LNA-modification ensures Tm-normalization). Synthesis was performed on a Paraflo microfluidic chip.
Contributor(s)
Zhong S , Li Q , He Z
Submission date
May 06, 2008
Last update date
May 08, 2008
Contact name
Li Qun
E-mail(s)
qli@sibs.ac.cn
Phone
+86 21 54924122
Organization name
Institute of plant physiology & ecology, SIBS, CAS
Department
National Key Laboratory of Plant Molecular Genetics
Street address
300 Fenglin Road
City
Shanghai
State/province
Shanghai
ZIP/Postal code
200032
Country
China
Samples (6)
GSM290566 , GSM290567 , GSM290568 , GSM290569 , GSM290570 , GSM290571
Series (1)
GSE11535
Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants
Data table header descriptions
ID
Probe_ID
Name of the detected RNA
SEQUENCE
Sequence of the detected RNA
Row
Position on array (row)
Column
Position on array (column)
Position
Position on array (single-cell format)
Description
Identifies Probe_ID
ORF
miRNA gene in Entrez Gene
SPOT_ID
Identifies rows other than miRNAs
Data table
ID
Probe_ID
SEQUENCE
Row
Column
Position
Description
ORF
SPOT_ID
1
empty
1
1
1-1:1
empty
empty
2
empty
1
2
1-1:2
empty
empty
3
empty
1
3
1-1:3
empty
empty
4
empty
1
4
1-1:4
empty
empty
5
empty
1
5
1-1:5
empty
empty
6
empty
1
6
1-1:6
empty
empty
7
empty
1
7
1-1:7
empty
empty
8
empty
1
8
1-1:8
empty
empty
9
empty
1
9
1-1:9
empty
empty
10
empty
1
10
1-1:10
empty
empty
11
empty
1
11
1-1:11
empty
empty
12
empty
1
12
1-1:12
empty
empty
13
empty
1
13
1-1:13
empty
empty
14
empty
1
14
1-1:14
empty
empty
15
empty
1
15
1-1:15
empty
empty
16
empty
1
16
1-1:16
empty
empty
17
empty
1
17
1-1:17
empty
empty
18
empty
1
18
1-1:18
empty
empty
19
empty
1
19
1-1:19
empty
empty
20
empty
1
20
1-1:20
empty
empty
Total number of rows: 3968 Table truncated, full table size 339 Kbytes .
Supplementary data files not provided