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Status
Public on Dec 01, 2008
Title
BTI DNA microarray for Actinosynema pretiosum
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Actinosynnema pretiosum
Manufacturer
Bioprocessing Technology Institute, Singapore
Manufacture protocol
1.PCR products were obtained from PCR reactions on genomic DNA of A. pretiosum. The primers were designed based on DNA sequences, AF453501 and U33059 as discussed in Yu et. al., 2002, PNAS, 99:7968. The DNA were arrayed in Abgene 384-well plates. Each well contains 100-150 ng/ul of DNA. 2. Rearray 10 µL into 384-well, Abgene 384-well microarray plates (AB-1056/w). 3. Print arrays using Biorad chipwriter pro, after print run is completed, let slides dry through passive evaporation in the arrayer.
Support
glass
Coating
poly-L-lysine
Contributor(s)
Chang A , Nissom PM
Submission date
Sep 11, 2008
Last update date
Oct 24, 2011
Contact name
Peter Nissom
E-mail(s)
pnissom@gmail.com
Organization name
Bioprocessing Technology Institute
Street address
20 Biopolis Way
City
Singapore
ZIP/Postal code
138668
Country
Singapore
Samples (24)
GSM319879 , GSM319880 , GSM319881 , GSM319882 , GSM319883 , GSM319884
GSM319885 ,
GSM319886 ,
GSM319887 ,
GSM319888 ,
GSM319889 ,
GSM319890 ,
GSM319891 ,
GSM319892 ,
GSM319893 ,
GSM319894 ,
GSM319895 ,
GSM319896 ,
GSM319897 ,
GSM319898 ,
GSM319899 ,
GSM319900 ,
GSM319901 ,
GSM319902
Series (1)
GSE12739
Transcriptional profile asm gene cluster of high Ansamitocin P3 producing mutants derived from A. pretiosum
Data table header descriptions
ID
GB_ACC
GenBank Accession number
SPOT_ID
Data table
ID
GB_ACC
SPOT_ID
16S rRNA_1
AB122775
GenBank accession: AB122775; 16S rRNA
16S rRNA_2
AB122775
GenBank accession: AB122775; 16S rRNA
16S rRNA_3
AB122775
GenBank accession: AB122775; 16S rRNA
16S rRNA_4
AB122775
GenBank accession: AB122775; 16S rRNA
aroF1
AF056968
GenBank accession: AF056968; aroF1
aroF2
AF056968
GenBank accession: AF056968; aroF2
asm1
AF453501
GenBank accession: AF453501; asm1
asm10
AF453501
GenBank accession: AF453501; asm10
asm12
AF453501
GenBank accession: AF453501; asm12
asm13
AF453501
GenBank accession: AF453501; asm13
asm14
AF453501
GenBank accession: AF453501; asm14
asm16
AF453501
GenBank accession: AF453501; asm16
asm17
AF453501
GenBank accession: AF453501; asm17
asm19
AF453501
GenBank accession: AF453501; asm19
asm2
AF453501
GenBank accession: AF453501; asm2
asm20
AF453501
GenBank accession: AF453501; asm20
asm21_1
AF453501
GenBank accession: AF453501; asm21
asm21_2
AF453501
GenBank accession: AF453501; asm21
asm21_3
AF453501
GenBank accession: AF453501; asm21
asm22
AF453501
GenBank accession: AF453501; asm22
Total number of rows: 69 Table truncated, full table size 3 Kbytes .
Supplementary file
Size
Download
File type/resource
GPL7284_array_list.txt.gz
3.8 Kb
(ftp) (http)
TXT
GPL7284_primer_sequences.txt.gz
2.6 Kb
(ftp) (http)
TXT