Center for Gene Expression Profiling (CGEP) at Boyce Thompson Institute of Plant Research
Manufacture protocol
TOM2 array were available from the Center for Gene Expression Profiling (CGEP) at Boyce Thompson Institute for Plant Research. TOM2 oligo array from tomato contain a total of 12800 elements or approximately 11860 unique elements. Sixty-nine oligonucleotides sequences from unigenes were synthesized by Operon (Operon biotechnologies, Hunstville, AL). Oligos were re-suspended in 12 uL of spotting buffer (3X SSC), and were spotted on Ultra Gaps Coated Slides (Corning, Lowell, MA) using a MicroGrid Pro arrayer (BioRobotics Inc., MA) with 32 MicroSpot2500 printing pins. Temperature and humidity inside the arrayer were maintained at 18-21°C and 35-45% RH, respectively. Oligos were fixed to the slide by 2-h incubation at 80°C.
Support
glass
Coating
aminosilane
Description
The array consist of 32 subgrids (4 meta-rows, 8 meta-columns). Each sub-grid contains 20 rows and 20 columns
