Individual cDNA inserts of diverse cDNA libraries were amplified with insert flanking primers. The PCR products were visualized on 1% agarose gels to test the quality and quantity of amplification, followed by purification with Multiscreen PCR plates (Millipore). The clean PCR products were reformatted in 384 well plates, dried and resuspended in 15 µl spotting buffer (3 x SSC, 1,5 M betain). Each PCR product was printed twice onto poly-L-lysine (Sigma) coated glass slides with a Microgrid II robot (BioRobotics). Slides were blocked with succinic anhydride and denatured in boiling water.
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