GEO help: Mouse over screen elements for information.
Status
Public on Oct 20, 2009
Title
Hox Tiling Array Agilent-018680 (ChIP)
Technology type
in situ oligonucleotide
Distribution
custom-commercial
Organism
Mus musculus
Manufacturer
Agilent
Manufacture protocol
Mouse hox regions A,B,D were identified and 60-mer probes were created that overlapped by 20 (probes were taken from both strands). The worst 3% of probes were removed to avoid cross-hybridization. Probes were uploaded to agilent using earray (http://earray.chem.agilent.com ) and arrays were ordered from agilent.
Submission date
Sep 24, 2009
Last update date
Oct 20, 2009
Contact name
Pengfei Wang
Organization name
Stowers Institute
Lab
Shilatifard
Street address
1000 E. 50th Street
City
Kansas City
State/province
MO
ZIP/Postal code
64110
Country
USA
Samples (10)
GSM456244 , GSM456245 , GSM456246 , GSM456247 , GSM456248 , GSM456249
Series (2)
GSE18258
Global analysis of H3K4 methylation
GSE18264
ChIP-chip with antibodies for histone 3 lysine 4 trimethylation, histone 3, and PolII in Mll1+/+ and Mll1-/- MEFs
Data table header descriptions
ID
ProbeName
probe id
chrom
chromosome
position
mid-point of probe in mm9 genome
type
one of hoxa, hoxb, hoxd, housekeeping gene (selected from Evidence Based Selection of Housekeeping Genes, Jonge et al., 2007), yy1/ies2 gene (selected based on appearance in data sets for YY1/Ies2 knockout in affymetrix arrays and qPCR), agilent control
SEQUENCE
probe sequence
RANGE_GB
RefSeq Assembly for mm9
RANGE_START
start coordinate on RefSeq assembly
RANGE_END
end coordinate on RefSeq assembly
RANGE_STRAND
strand to which probe corresponds
SPOT_ID
identifies type of probe
Data table
ID
ProbeName
chrom
position
type
SEQUENCE
RANGE_GB
RANGE_START
RANGE_END
RANGE_STRAND
SPOT_ID
1
MmCGHBrightCorner
agilent control
control
2
DarkCorner
agilent control
control
3
DarkCorner
agilent control
control
4
DarkCorner
agilent control
control
5
DarkCorner
agilent control
control
6
DarkCorner
agilent control
control
7
DarkCorner
agilent control
control
8
DarkCorner
agilent control
control
9
DarkCorner
agilent control
control
10
DarkCorner
agilent control
control
11
DarkCorner
agilent control
control
12
e_chr2.74595287.W
2
74595287
hoxd
GATTCTCATCACCCGCATAGCAGTTAACAACTGTATCTCCAGTTCCAGGGGACATGATAC
NC_000068.6
74595287
74595346
+
hoxd
13
e_chr6.51986988.W
6
51986988
hoxa
CTCAGATTCCCTAAATAAATTCTAATAAATAGGAAAGAGGATGTGGAAGCCTGCTAACCC
NC_000072.5
51986988
51987047
+
hoxa
14
e_chr6.51994988.W
6
51994988
hoxa
TTTGGTTCAAGTACAAGTGTTAGGGAATGCAGGGTGCAGCACTACCTAATTACAACGGCT
NC_000072.5
51994988
51995047
+
hoxa
15
e_chr2.74531168.C
2
74531168
hoxd
AAATGATCCCTGGGTTCAAGGCAAAAACTAGGGCTGCAAATCTGAGCCAAAGAAAAGCGG
NC_000068.6
74531109
74531168
-
hoxd
16
e_cnwy_trim29_chr9.43119134.W
9
43119134
yy1/ies2 gene
CCTGAAGTCAGGGGAGGGGAAGAGTGGCCTATTTTCCAGCAATGAGTGGCGCAGGCCTAT
NC_000075.5
43119134
43119193
+
yy1/ies2 gene
17
e_chr11.96249038.C
11
96249038
hoxb
ACCAGGGCAACATTCCCCAACCTATGGGGTCTTGATCCTTCTGAGGGTCACATATCAGAT
NC_000077.5
96248979
96249038
-
hoxb
18
e_cnwy_lnx1_chr5.75071489.W
5
75071489
yy1/ies2 gene
GAATTGTGAACATGACCTTATTTAGATAAAATGCGTTAGGACTGAAATTAAACTAGGCTA
NC_000071.5
75071489
75071548
+
yy1/ies2 gene
19
e_chr6.52082108.W
6
52082108
hoxa
AAAATGATGAAATACTATCTCAAAAAAAAAGTCAAATTGGTATAGACCTAATGTTCTCTT
NC_000072.5
52082108
52082167
+
hoxa
20
e_chr6.52185424.C
6
52185424
hoxa
AGGCCAGCTCCAGGCCCAGAAATGTATAGGATAGCTAGCCTGCTACAGGGCCCGTTTAAA
NC_000072.5
52185365
52185424
-
hoxa
Total number of rows: 45220 Table truncated, full table size 6295 Kbytes .
Supplementary data files not provided