CustomArray uses a specially modified “CMOS” semiconductor to direct the molecular assembly of a specific sequence of DNA bases in response to a digital command. Each feature on the array (a microelectrode) is digitally addressed to selectively generate acid, by means of an electrochemical reaction which, in-turn, controls the detritylation reaction during phosphoramidite synthesis. A software program is used to control the microelectrodes, and thus, the detritylation pattern applied on the chip during synthesis.
Description
High-density (90k) in situ synthesized microarray for Ovis aries, designed by means of a pipeline of software instruments that, starting from unannotated redundant EST sequences deposited at NCBI, selects oligonucleotides suitable for in situ generation on chip. The chip carries 21,743 non-redundant Ovis aries features in quadruplicate, 73.4% of which are fully annotated and correspond to 10,190 genes, thus representing a good coverage of the sheep genome.
Bongiorni S., Chillemi G., Prosperini G., Bueno S., Valentini A., Pariset L. A Tool for Sheep Product Quality: Custom Microarrays from Public Databases. Nutrients. 2009; 1(2):235-250. doi:10.3390/nu1020235
