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Platform GPL9730 Query DataSets for GPL9730
Status Public on Mar 01, 2010
Title CSIR Fungi 20090901 96 v1.0
Technology type spotted oligonucleotide
Distribution non-commercial
Organisms Aspergillus; Aspergillus amstelodami; Aspergillus clavatus; Aspergillus niger; Penicillium; Fusarium; Fusarium oxysporum; Fusarium sporotrichioides; Fusarium acuminatum; Fusarium graminearum; Alternaria alternata; Penicillium expansum; Talaromyces islandicus; Fusarium avenaceum; Aspergillus carbonarius; Fusarium subglutinans; Aspergillus versicolor; Fusarium cerealis; Fusarium equiseti; Penicillium fellutanum; Penicillium corylophilum; Fusarium verticillioides
Manufacturer CSIR and ACGT Microarray Facility, University of Pretoria, Pretoria, South Africa
Manufacture protocol 1. Oligonucleotide probes ranging in size from 16 - 23 bases were transferred to Genetix X7022 384-well plates for arraying.
2. Each well contained a final concentration of 20 µM oligonucleotide probe in 50% DMSO spotting solution (v/v).
3. Between printing runs the plates were stored at -20°C.
4. Prior to printing the plates were incubated at room temperature for 30 min to aid temperature equilibration.
5. Plates were centrifuged at 2000 x g for 5 minutes prior to printing.
6. Printing was performed on the Molecular Dynamics Generation III spotter.
7. Seals were removed and plates placed in the spotter immediately prior to initiating the printing process to prevent unnecessary evaporation.
8. A relative humidity of 50% was maintained in the spotter cabinet during printing runs.
9. Following completion of the printing run the plates were sealed with a new Abgene adhesive plate seal and stored at -20°C.
 
Description Forty food-borne fungi
 
Submission date Nov 30, 2009
Last update date Mar 01, 2010
Contact name Eugenia Barros
E-mail(s) slezar@csir.co.za
Phone 0027-12-8413221
Fax 0027-12-8413651
Organization name CSIR
Department Building 20
Lab Biosciences
Street address Meiring Naude Rd
City Pretoria
State/province Gauteng
ZIP/Postal code 0001
Country South Africa
 
Samples (120) GSM476355, GSM476356, GSM476357, GSM476358, GSM476359, GSM476360 
Series (1)
GSE19227 Oligonucleoteide microarray for the identification of potential mycotoxigenic fungi

Data table header descriptions
ID Clone identifier
POSITION (BLOCK, ROW, COLUMN) Position of oligonucleotide probe
OLIGO UID Oligonucleotide probe identifier
SEQUENCE Sequence of oligonucleotide probe
ORGANISM organism
DESCRIPTION Description of probe
SPOT_ID spot identifier

Data table
ID POSITION OLIGO UID SEQUENCE ORGANISM DESCRIPTION SPOT_ID
1 1_1_1 LanSpoR1 TACAAGAAGAGCGTGGCGATAT Fusarium sporotrichioides LanSpoR1
2 1_1_2 ITSF CAACTCCAAACCCCTGTGA Fusarium genus ITSF
3 1_1_3 AcarR CCCGAGGCAGAGATG Aspergillus carbonarius AcarR
4 1_1_4 EurAR TGGTTAAAAGATTGGTTGCGA Eurotium amstelodami EurAR
5 1_1_5 ITS4 TCCTCCGCTTATTGATATGC Internal Transcribed Region ITS4
6 1_1_6 AClF ATTCGGAAACCUGCTCAGTACG Aspergillus clavatus AClF
7 1_1_7 53-6R GGCCGTTTACCTGGCTTCTT Fusarium moniliforme/ verticilliodes 53-6R
8 1_1_8 FaF CAAGCATTGTCGCCACTCTC Fusarium avenaceum FaF
9 1_1_9 FGDR CGCCAATCAATTTGAGGAACG Fusarium oxysporum FGDR
10 1_1_10 AclaR CGTGTTGTACAACGTTTA Aspergillus clavatus AclaR
11 1_1_11 ITS1 TCCGTAGGTGAACCTGCG Internal Transcribed Region ITS1
12 1_1_12 FGDR CGCCAATCAATTTGAGGAACG Fusarium oxysporum FGDR
13 1_1_13 negative control control
14 1_1_14 ITSF CAACTCCAAACCCCTGTGA Fusarium genus ITSF
15 1_1_15 PenIsF CGAGTGCGGGTTCGACA Penicillium islandicum PenIsF
16 1_1_16 ITS60 ACAGAGCGGTGACA Penicillium spp. ITS60
17 1_1_17 FaF CAAGCATTGTCGCCACTCTC Fusarium avenaceum FaF
18 1_1_18 Tri5R AATTCTCCATCTGACCATCCAG All trichothecenes Tri5R
19 1_1_19 negative control control
20 1_1_20 PenFeF CTGAGTGCGGGCCCTCT Penicillium fellutanum PenFeF

Total number of rows: 3072

Table truncated, full table size 190 Kbytes.




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Supplementary data files not provided
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