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Status
Public on Dec 15, 2009
Title
Plants 4K miRNA LC_MRA-1038_miRNAPlants_10.0 + Bn_PsRNAs
Technology type
in situ oligonucleotide
Distribution
custom-commercial
Organisms
Physcomitrium patens ; Pinus taeda ; Populus trichocarpa ; Arabidopsis thaliana ; Brassica napus ; Glycine max ; Medicago truncatula ; Oryza sativa ; Saccharum officinarum ; Sorghum bicolor ; Triticum aestivum ; Zea mays ; Selaginella moellendorffii
Manufacturer
LC Sciences
Manufacture protocol
Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/ ) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.
Support
silicon
Description
Plant miRNA microarray with 100% coverage of mature miRNAs listed in miRBase version 10.0 and additional custom small RNA sequences from Brassica napus phloem sap (Bn_PsRNAs)
Web link
http://www.LCsciences.com
Contributor(s)
Buhtz A , Kehr J
Submission date
Dec 02, 2009
Last update date
Dec 15, 2009
Contact name
Anja Buhtz
E-mail(s)
anja.buhtz@upm.es
Organization name
Centro de Biotecnologia y Genomica de Plantas
Lab
280
Street address
Campus de Montegancedo, M40,km38
City
Madrid
ZIP/Postal code
28223
Country
Spain
Samples (3)
GSM507489 , GSM507490 , GSM507491
Series (1)
GSE20263
Small RNAs in different tissues and phloem of oilseed rape under nutrient stress conditions
Data table header descriptions
ID
Probe_ID
Name of the probe on the chip
Target Sequence
Target sequence (5’ to 3’) of the probe
Row
Row of the probe position on the chip
Column
Column of the probe position on the chip
Group
General function of the particular probe on the chip: experimental/control
Comment
Information of the sequence: mature miRNA/custom sequence
SEQUENCE
sequence of probe
miRNA_ID
Data table
ID
Probe_ID
Target Sequence
Row
Column
Group
Comment
SEQUENCE
miRNA_ID
1
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
8
13
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
2
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
78
3
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
3
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
98
1
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
4
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
109
23
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
5
Bn_PsRNA_01
UGAACAAGGUUUCCCAGAUGC
122
1
experimental
Custom Sequence
GCATCTGGGAAACCTTGTTCA
PsRNA_01
6
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
3
19
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
7
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
73
9
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
8
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
93
31
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
9
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
104
29
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
10
Bn_PsRNA_02
UUGAAAAACAGAGACAAUAAAGGC
121
31
experimental
Custom Sequence
GCCTTTATTGTCTCTGTTTTTCAA
PsRNA_02
11
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
2
16
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
12
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
72
6
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
13
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
80
21
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
14
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
103
26
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
15
Bn_PsRNA_03
CCAUGGGAAUCGAACUAGGGGUGC
104
21
experimental
Custom Sequence
GCACCCCTAGTTCGATTCCCATGG
PsRNA_03
16
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
8
17
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
17
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
78
7
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
18
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
98
21
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
19
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
109
27
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
20
Bn_PsRNA_04
AAUAGGGAGGGCAAGGAGUGC
122
21
experimental
Custom Sequence
GCACTCCTTGCCCTCCCTATT
PsRNA_04
Total number of rows: 3540 Table truncated, full table size 366 Kbytes .
Supplementary file
Size
Download
File type/resource
GPL9736_sequences for Platform_miRNA_release_10.0_14Dec09.txt.gz
12.9 Kb
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