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Platform GPL9751 Query DataSets for GPL9751
Status Public on Dec 09, 2009
Title Cavalieri-Yeast-ORF-7k Array
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Saccharomyces cerevisiae
Manufacturer Bauer Laboratory, FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
Manufacture protocol Yeast microarrays were constructed using the amplifying to levels required for preparation of DNA microarrays by PCR (27) a set of clones containing 6218 verified ORFs of the yeast genome obtained from Research Genetics (Huntsville, AL). Some of the longer ORFs were amplified with the GIBCO/BRL Elongase Amplification Kit (Life Technologies, Rockville, MD), using 40 cycles of 1 min at 95 °C denaturation, 1 min at 55 °C annealing, and 10 min at 68 °C elongation. We obtained an amplified product confirmed by agarose gel electrophoresis for 98% of the ORFs. The amplified DNA was precipitated with isopropanol, washed with 70% EtOH, and resuspended in 25 μl Micro Spotting Solution (Telechem, Sunnyvale, CA). The DNA was spotted on CMT-GAPS amino-silane-coated glass slides (Corning), using a microarraying robot with a 16-pin head constructed from a design by Patrick O. Brown (http://cmgm.stanford.edu/pbrown/).
Support glass
Coating aminosilane
 
 
Contributor(s) Cavalieri D, Morneau K
Submission date Dec 04, 2009
Last update date Dec 09, 2009
Contact name Irene Stefanini
E-mail(s) stefanini.irene@gmail.com
Organization name University of Turin
Street address via Accademia Albertina
City Torino
ZIP/Postal code 10123
Country Italy
 
Samples (6) GSM480232, GSM480249, GSM480250, GSM480251, GSM480252, GSM480253
Series (1)
GSE19331 Transcriptional analysis of the effects of 089 on a panel of yeast strains

Data table header descriptions
ID
Block collection of spots
Row row position of the spot in the selected block
Column column position of the spot in the selected block
Name common name of the ORF
ORF locus tag
SPOT_ID

Data table
ID Block Row Column Name ORF SPOT_ID
YAL068C 1 1 1 YAL068C
YAR002C-A 1 1 2 ERP1 YAR002C-A
YAR003W 1 1 3 YAR003W
YAR008W 1 1 4 SEN34 YAR008W
YAR010C 1 1 5 YAR010C
YAR015W 1 1 6 ADE1 YAR015W
YAR047C 1 1 7 YAR047C
YAR053W 1 1 8 YAR053W
YAR061W 1 1 9 YAR061W
YAR064W 1 1 10 YAR064W
YAR068W 1 1 11 YAR068W
YAR070C 1 1 12 YAR070C
YBL009W 1 1 13 YBL009W
YBL011W 1 1 14 SCT1 YBL011W
YBL013W 1 1 15 FMT1 YBL013W
YBL015W 1 1 16 ACH1 YBL015W
YBL017C 1 1 17 PEP1 YBL017C
YBL019W 1 1 18 APN2 YBL019W
YBL033C 1 1 19 RIB1 YBL033C
YBL035C 1 1 20 POL12 YBL035C

Total number of rows: 7056

Table truncated, full table size 193 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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