|
| Status |
Public on Jun 15, 2017 |
| Title |
Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome (H4R3me2s ChIP-Seq) |
| Organism |
Human gammaherpesvirus 8 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The objective of this study was to identify the symmetric methylation sites on KSHV genome during latency and lytic reactivation
|
| |
|
| Overall design |
KSHV infected, TRExBCBL1-RTA cells were induced with doxycycline for 12h to trigger the lytic reactivation, cells were crosslinked and subjected for chromatin isolation and sonication. ChIP assay was performed using anti-H4R3sym antibody, Control IgG antibody, or Histone H4 antibody.
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| |
|
| Contributor(s) |
Verma SC |
| Citation(s) |
28678843 |
| Submission date |
Jun 14, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Subhash C Verma |
| E-mail(s) |
scverma@medicine.nevada.edu
|
| Phone |
775-682-6743
|
| Organization name |
University of Nevada, Reno
|
| Department |
Microbiology and Immunology
|
| Lab |
Verma Lab
|
| Street address |
1664 N Virginia Street, MS 320
|
| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23366 |
Illumina NextSeq 500 (Human gammaherpesvirus 8) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE98058 |
Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome |
|
| Relations |
| BioProject |
PRJNA390547 |
| SRA |
SRP109223 |
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