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| Status |
Public on Jul 06, 2017 |
| Title |
An integrated computational-experimental approach reveals Yersinia pestis genes essential across a narrow or a broad range of environmental conditions |
| Organism |
Yersinia pestis |
| Experiment type |
Other
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| Summary |
Using a library of over 1 million Y. pestis CO92 random mutants and transposon-directed insertion site sequencing, we identified 530 essential genes when the bacteria were cultured at 28oC. When the library of mutants was subsequently cultured at 37oC we identified 19 genes that were essential at 37oC but not at 28oC, including genes which encode proteins that play a role in enabling functioning of the type III secretion and in DNA replication and maintenance.
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| Overall design |
100 bp single end reads on an Illumina HiSeq 2500 standard mode
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| Contributor(s) |
Titball RW, Senior NJ, Sasidharan K, Saint RJ, Scott AE, Sarkar-Tyson M, Ireland PM, Bullifent HL, Moore K, Oyston PC, Atkins TP, Atkins HS, Soyer OS, Yang ZR |
| Citation(s) |
28732479 |
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| Submission date |
Jun 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
ZhengRong Yang |
| E-mail(s) |
z.r.yang@exeter.ac.uk
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| Phone |
0044 1392 263448
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| Organization name |
University of Exeter
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| Street address |
Prince of Wales Road
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| City |
Exeter |
| ZIP/Postal code |
EX4 4PS |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL23606 |
Illumina HiSeq 2500 (Yersinia pestis) |
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| Samples (7)
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| GSM2674959 |
Yersinia pestis transposon mutant library cultured at 28ºC (replicate 1) |
| GSM2674960 |
Yersinia pestis transposon mutant library cultured at 28ºC (replicate 2) |
| GSM2674961 |
Yersinia pestis transposon mutant library cultured at 28ºC (replicate 3) |
| GSM2674962 |
Yersinia pestis transposon mutant library cultured at 37ºC (replicate 1b) |
| GSM2674963 |
Yersinia pestis transposon mutant library cultured at 37ºC (replicate 1a) |
| GSM2674964 |
Yersinia pestis transposon mutant library cultured at 37ºC (replicate 2) |
| GSM2674965 |
Yersinia pestis transposon mutant library cultured at 37ºC (replicate 3) |
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| Relations |
| BioProject |
PRJNA391106 |
| SRA |
SRP109777 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100226_RAW.tar |
168.2 Mb |
(http)(custom) |
TAR (of RD) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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