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| Status |
Public on Jan 10, 2008 |
| Title |
The acute effects of methoxyacetic acid exposure on testis gene expression |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We evaluated the possible mechanisms by which 2-methoxyacetic acid (MAA) disrupts spermatogenesis using high density microarrays. Levels of mRNA transcripts were determined in total RNA isolated from testes of MAA-treated (650 mg/kg i.p.) or concurrent control rats sacrificed 4, 8, 12 or 24 hrs post exposure (PE).
Keywords: Toxicology, Time Course,
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| Overall design |
Sexually mature Sprague Dawley male rats were treated i.p. with 650 mg/kg of 2-methoxyacetic acid (CAS# 625-45-6). Treated animals were sacrificed, along with concurrent control animals 4, 8 12 or 24 hr post exposure (n=6 per treatment or control per time point, 48 total animals). Agilent 20K oligonucleotide microarrays were used to examine the levels of transcripts in whole testis total RNA. Universal rat RNA was used as reference.
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| Contributor(s) |
Wade MG, Yauk CL, Williams A, Kawata AK |
| Citation(s) |
18199887 |
| Submission date |
Dec 27, 2007 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Mike G Wade |
| E-mail(s) |
Mike_Wade@hc-sc.gc.ca
|
| Phone |
613 946 5127
|
| Fax |
613 957 8800
|
| Organization name |
Health Canada
|
| Department |
Environmental Health Science & Research Bureau
|
| Lab |
Reproductive Toxicology
|
| Street address |
50 Columbine Driveway
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1A 0K9 |
| Country |
Canada |
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| Platforms (1) |
| GPL890 |
Agilent-011868 Rat Oligo Microarray G4130A (Feature Number version) |
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| Samples (47)
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| Relations |
| BioProject |
PRJNA108591 |
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