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Series GSE100491 Query DataSets for GSE100491
Status Public on May 16, 2019
Title Transcriptomics analysis of gene expression in control, Prrc2a conditional knockout, and Fto transgene mouse brain sample
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA was isolated from hippocampus sample from 4 weeks old prrc2a flox/flox and prrc2a flox/flox;Olig2-cre or 4 weeks old wt and Fto Tg micemice using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer's protocol.The libraries were sequenced using HiSeq3000 (Illumina) in paired-read mode, creating reads with a length of 150 bp. Sequencing chemistry v2 (Illumina) was used.
Overall design Examination of gene expressive levels in
prrc2a flox/flox and prrc2a flox/flox; nestin-cre mice.
control and Fto transgene mice.
Contributor(s) Wu R, Li A, Sun B, Yuan Z, Yang Y
Citation(s) 30514900
Submission date Jun 26, 2017
Last update date Jul 25, 2021
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (6)
GSM2684967 Prrc2a WT RNA-seq
GSM2684968 Prrc2a cKO RNA-seq
GSM2803005 prrc2a WT mice_hippocampus_RNA-seq
This SubSeries is part of SuperSeries:
GSE104541 Control, Prrc2a conditional knockout, and Fto transgene mouse brain study
BioProject PRJNA391946
SRA SRP110525

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Supplementary file Size Download File type/resource
GSE100491_RAW.tar 880.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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