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| Status |
Public on Sep 05, 2019 |
| Title |
Notch signaling is required for induction of an innate T cell development program in human thymus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
CD4 CD8 double positive (DP) thymocytes progressively shut down Notch signaling after β-selection allowing progression of the DP program and positive selection of conventional CD4 and CD8 single positive T cells. In contrast, innate CD8+ T cells are selected from DP precursors shortly after β-selection. Here, we investigated whether notch signaling is required for the initiation of the innate T cell developmental program upon TCR engagement. We show that, after β-selection, Notch activation limits progression to late DP cells and favors the development of TCRαβ/CD3 expressing CD8dim early DP progenitors. TCR engagement in these Notch dependent TCRαβ+ early DP precursors does not induce prominent apoptosis, but rather induces T-bet expression by an mTOR-dependent mechanism. Our findings indicate that Notch signaling drives the launch of an innate effector program in response to TCR agonist selection in the thymus.
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| Overall design |
Three samples are analyzed. The first sample comes from native thymus tissue. CD34+ postnatal thymocytes were transduced to express either TCRalpha (second sample) or TCRbeta (third sample). Subsequently, the transduced thymocytes were cultured on OP9-DL1 cells. When the cells were double positive, the cells were harvested sorted for immature CD3+ DP cells transgenic for the TCR.
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| Contributor(s) |
Verstichel G, Vandekerckhove B |
| Citation missing |
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| Submission date |
Jun 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Rekin's Janky |
| E-mail(s) |
Nucleomics.Bioinformatics@vib.be
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| Organization name |
VIB
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| Department |
Nucleomics Core
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| Street address |
Herestraat 49 Box 816
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| City |
Leuven |
| ZIP/Postal code |
B-3000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA392169 |
