NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE100519 Query DataSets for GSE100519
Status Public on Sep 05, 2019
Title Notch signaling is required for induction of an innate T cell development program in human thymus
Organism Homo sapiens
Experiment type Expression profiling by array
Summary CD4 CD8 double positive (DP) thymocytes progressively shut down Notch signaling after β-selection allowing progression of the DP program and positive selection of conventional CD4 and CD8 single positive T cells. In contrast, innate CD8+ T cells are selected from DP precursors shortly after β-selection. Here, we investigated whether notch signaling is required for the initiation of the innate T cell developmental program upon TCR engagement. We show that, after β-selection, Notch activation limits progression to late DP cells and favors the development of TCRαβ/CD3 expressing CD8dim early DP progenitors. TCR engagement in these Notch dependent TCRαβ+ early DP precursors does not induce prominent apoptosis, but rather induces T-bet expression by an mTOR-dependent mechanism. Our findings indicate that Notch signaling drives the launch of an innate effector program in response to TCR agonist selection in the thymus.
 
Overall design Three samples are analyzed. The first sample comes from native thymus tissue. CD34+ postnatal thymocytes were transduced to express either TCRalpha (second sample) or TCRbeta (third sample). Subsequently, the transduced thymocytes were cultured on OP9-DL1 cells. When the cells were double positive, the cells were harvested sorted for immature CD3+ DP cells transgenic for the TCR.
 
Contributor(s) Verstichel G, Vandekerckhove B
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 27, 2017
Last update date Jul 25, 2021
Contact name Rekin's Janky
E-mail(s) Nucleomics.Bioinformatics@vib.be
Organization name VIB
Department Nucleomics Core
Street address Herestraat 49 Box 816
City Leuven
ZIP/Postal code B-3000
Country Belgium
 
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (3)
GSM2685849 4SPII KT
GSM2685850 TCRaDP
GSM2685851 TCRbDP
Relations
BioProject PRJNA392169

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100519_RAW.tar 26.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap