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| Status |
Public on Nov 04, 2017 |
| Title |
The transcriptional response to unilateral nephrectomy with and without ischemia-reperfusion injury |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptome analysis was done after warm renal ischemia-reperfusion injury (IRI) in a rat model. Earlier studies have shown a protective effect of prior unilateral nephrectomy (UNx) against IRI in the remaining, contralateral kidney compared to a non-neprectomized control group. We aimed at identifying the underlying molecular mechanisms. We used the Affymetrix Clariom D array (formerly known as RTA 1.0 st.)
Array data was processed in the Affymetrix Console Software.
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| Overall design |
We analysed kidney tissue (left) from male Wistar rats. Number of animals from each group, n = 3. Wistar rats were randomized to either UNx or sham UNx immediately prior to 37 minutes of unilateral renal artery clamping or sham operation under sevoflurane anesthesia. Groups: sham (2 normal kidneys), UNx (1 remaining kidney), IR (1 ischemic kidney, 1 normal kidney), IR+UNx (1 ischemic kidney). MRI was performed 24 hours after reperfusion. Blood and renal tissue were harvested. RNA was isolated for microarray analysis.
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| Contributor(s) |
Kierulf-Lassen C, Nielsen PM, Qi H, Damgaard M, Laustsen C, Pedersen M, Birn H, Nørregaard R, Jespersen B |
| Citation(s) |
29267404 |
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| Submission date |
Jun 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Casper Kierulf Lassen |
| E-mail(s) |
cklassen@dadlnet.dk
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| Organization name |
Aarhus University
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| Department |
Department of Clinical Medicine
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| Street address |
Palle Juul-Jensens Boulevard 99
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| City |
Aarhus N |
| ZIP/Postal code |
8200 |
| Country |
Denmark |
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| Platforms (1) |
| GPL23629 |
[RTA-1_0] Affymetrix Rat Transcriptome Array 1.0 [probe set (exon) CSV version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA392078 |
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