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| Status |
Public on May 28, 2018 |
| Title |
Comparison of CD4+ and CD8+ T cells from spleen of mice raised in standard or in enriched environment. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
C57BL6/J female mice were raised either in standard environment (SE) for 8 weeks after birth or were put in enriched environment (EE) at postnatal 4 weeks up to 8 weeks old. Mice were sacrificed at 8 weeks and spleen were harvested. CD4+ and CD8+ T cells were sorted by FACS and CD4+ or CD8+ T were profiled using a low-input protocol adapted from single-cell RNAseq.
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| Overall design |
21 samples were profiled: for CD8+ T Cells, n=6 in EE and n=4 in SE. For CD4+ T Cells, N=6 in EE and N=5 in SE.
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| Contributor(s) |
Guyon A, Zarif H, Arguel M, Lebrigand K, Paquet A, Barbry P |
| Citation(s) |
29950983 |
| |
| Submission date |
Jun 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA392225 |
| SRA |
SRP110657 |
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