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Status |
Public on Dec 31, 2020 |
Title |
Allele-specific genome editing using the CRISPR/Cas9 system phenocopies alopecia areata symptomatic patched hair loss in mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Using CRISPR/Cas9 for allele-specific genome editing we phenocopied AA symptomatic patched hair loss in mice engineered to carry the Cchcr1 risk allele.
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Overall design |
Fluorescent cRNA synthesis derived from eight skin RNAs was performed using Agilent’s Low RNA Input Linear Amplification kit (Agilent Technology) and subjected to DNA microarray analysis using single-color microarray-based gene-expression analysis (SurePrint G3 Mouse GE Ver 2.0, 8 x 60K, Agilent Technology). All procedures were performed according to the manufacturer’s instructions.
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Contributor(s) |
Oka A, Ikeda S |
Citation missing |
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Submission date |
Jun 28, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Akira Oka |
E-mail(s) |
oka246@is.icc.u-tokai.ac.jp
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Phone |
463931121
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Organization name |
Tokai university
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Street address |
143 Shimokasuya
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City |
isehara |
State/province |
Kanagawa |
ZIP/Postal code |
259-1193 |
Country |
Japan |
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Platforms (1) |
GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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Samples (8)
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Relations |
BioProject |
PRJNA392323 |