|
| Status |
Public on Jul 01, 2018 |
| Title |
Identification of genes associated with TGF-beta/Smad3-mediated macrophage-myofibroblast transition (MMT) by single-cell RNA sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we conducted single-cell RNA sequencing (scRNA-Seq) to identify the Smad3-dependent transcriptomes related to MMT in TGF-beta1-treated bone marrow-derived macrophages in vitro, where Smad3-KO group serves as a negative control.
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| |
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| Overall design |
The bone marrow macrophages of Smad3-WT or -KO mice were stimulated with 5ng/ml of TGF-beta1 for 5 days. Then the a-SMA negative or positive F4/80-expressing cells were flow sorted and subjected to single-cell RNA sequencing analysis.
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| |
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| Contributor(s) |
Tang PM, Zhou S, Huang XR, To KF, Ma RC, Lee TL, Lan HY |
| Citation(s) |
29042082 |
| |
| Submission date |
Jun 30, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hui-Yao Lan |
| E-mail(s) |
hylan@cuhk.edu.hk
|
| Organization name |
The Chinese University of Hong Kong
|
| Department |
Li Ka Shing Institute of Health Sciences, Department of Medicine & Therapeutics, CUHK-Shenzhen Research Institute
|
| Lab |
Room 601, Li Ka Shing Medical Sciences Building
|
| Street address |
Prince of Wales Hospital, Shatin, N.T.
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
Hong Kong |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (17)
|
| GSM2691825 |
Smad3-wildtype bone marrow-derived macrophage [S3WT-M1] |
| GSM2691826 |
Smad3-wildtype bone marrow-derived macrophage [S3WT-M2] |
| GSM2691827 |
Smad3-wildtype bone marrow-derived macrophage [S3WT-M3] |
| GSM2691828 |
Smad3-wildtype bone marrow-derived macrophage [S3WT-M4] |
| GSM2691829 |
Smad3-wildtype bone marrow-derived macrophage [S3WT-M5] |
| GSM2691830 |
TGF-beta1-treated Smad3-wildtype bone marrow-derived macrophage [S3WT-MMT1] |
| GSM2691831 |
TGF-beta1-treated Smad3-wildtype bone marrow-derived macrophage [S3WT-MMT2] |
| GSM2691832 |
TGF-beta1-treated Smad3-wildtype bone marrow-derived macrophage [S3WT-MMT3] |
| GSM2691833 |
TGF-beta1-treated Smad3-wildtype bone marrow-derived macrophage [S3WT-MMT4] |
| GSM2691834 |
Smad3-knockout bone marrow-derived macrophage [S3KO-M1] |
| GSM2691835 |
Smad3-knockout bone marrow-derived macrophage [S3KO-M2] |
| GSM2691836 |
Smad3-knockout bone marrow-derived macrophage [S3KO-M3] |
| GSM2691837 |
Smad3-knockout bone marrow-derived macrophage [S3KO-M4] |
| GSM2691838 |
Smad3-knockout bone marrow-derived macrophage [S3KO-M5] |
| GSM2691839 |
TGF-beta1-treated Smad3-knockout bone marrow-derived macrophage [S3KO-MMT1] |
| GSM2691840 |
TGF-beta1-treated Smad3-knockout bone marrow-derived macrophage [S3KO-MMT2] |
| GSM2691841 |
TGF-beta1-treated Smad3-knockout bone marrow-derived macrophage [S3KO-MMT3] |
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| Relations |
| BioProject |
PRJNA392697 |
| SRA |
SRP110831 |
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