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| Status |
Public on Jul 01, 2018 |
| Title |
Gene expression profiles of Fir1 (RNAi) animals |
| Organism |
Schmidtea mediterranea |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this study was to identify Fir1 downstream genes that were required for local proliferation. Fir1(RNAi) animals displayed reduced local proliferation near the wound,while the proliferation far from the wound was not decreased after Fir1 RNAi. The regenerating pieces following Fir1 RNAi were expected to only show decreased levels of the genes near the wound relative to control RNAi animals.
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| Overall design |
Animals were administrated by Fir1 dsRNA for 8 days, amputated after pharynx. 48 hours later, RNA from the region adjacent to the wound(part1) and the region far from the wound(part2) in Fir1(RNAi) tail pieces was harvested with Trizol. Two biological replicates were used. Then the RNA was hybridized on a microarray with RNA from control RNAi animals.
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| Contributor(s) |
Han X, Jing Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Qing Jing |
| Organization name |
Shanghai Institutes for Biological Sciences, CAS
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| Department |
Institute of Health Sciences
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| Lab |
Jing
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| Street address |
320 Yue Yang Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL23660 |
Agilent-073108 Schmidtea mediterranea 8*60K Smed_microarray_Jing_xshan |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA393175 |
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