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Series GSE101471 Query DataSets for GSE101471
Status Public on Jul 15, 2017
Title Transcriptome of Escherichia coli cells in coculture (Ile auxotroph and Leu auxotroph)
Platform organism Escherichia coli
Sample organism Escherichia coli K-12
Experiment type Expression profiling by genome tiling array
Summary We conducted experimental evolution of E. coli a mutual cross-feeding coculture, composed of two genetically-engineered Escherichia coli populations, I- and L-, which lack genes for biosynthesis of Ile (ilvE gene) and Leu (leuB gene), respectively. These two populations grew in a coculture by complementing required nutrients each other. After 883 generations of evolution experiments, we measured evolutionary changes in transcriptome, and we found ABC transporters for the branched chain amino acids (Ile, Leu, and Val) were significantly increased in both I- and L-. Also, we got other various results that would be interesting to discuss.
Overall design In total, 20 samples.
Contributor(s) Hosoda K, Ono N, Suzuki S
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Submission date Jul 14, 2017
Last update date Jul 25, 2021
Contact name Kazufumi Hosoda
Organization name Osaka university
Street address Yamadaoka 1-5
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (1)
GPL13336 Affymetrix Escherichia coli full sequence array (EcFS) [EcFS_1, EcFS_2, and EcFS_3]
Samples (20)
GSM2704094 IA-starve
GSM2704095 LA-starve
GSM2704096 IE-starve
BioProject PRJNA394521

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE101471_Ecoli_co_results.xlsx 2.9 Mb (ftp)(http) XLSX
GSE101471_RAW.tar 998.1 Mb (http)(custom) TAR (of CEL)
Processed data are available on Series record

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