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Series GSE101635 Query DataSets for GSE101635
Status Public on Apr 04, 2018
Title PTEN loss and expression of the TMPRSS2/ERG fusion gene transform prostatic epithelial cells via enhanced FGF signaling
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To determine if ERG expression and PTEN loss were sufficient to transform prostate epithelial cells we constructed cell lines with expression of the TMPRSS2/ERG fusion gene (TE), stable knockdown of PTEN with shRNA (PTEN KD) or both alterations (PTEN KD/TE) using the PNT1A cell line. To determine what gene expression changes are associated the phenotypic changes between the cell line experimental groups, we carried expression microarray studies using Agilent 60K expression microarrays. RNAs from all four cell lines were analyzed in duplicate and probes with ≥ 1.4-fold or ≤0.7-fold relative to control cells identified. Our results demonstrated that PTEN loss and expression of the TMPRSS2/ERG fusion gene transform prostatic epithelial cells via enhanced FGF signaling.
 
Overall design Microarray analyses of four cell lines (PA, PA-TE, PA-PTEN KD and PA-TE+PTEN KD).
 
Contributor(s) Shao L, Wang J, Zhang Y, Creighton C, Ittmann M
Citation(s) 29581856
Submission date Jul 19, 2017
Last update date Jul 25, 2021
Contact name Chad Creighton
E-mail(s) creighto@bcm.tmc.edu
Organization name Baylor College of Medicine
Department Biostatistics, Ducan Cancer Center
Street address One Baylor Plaza, Mail Stop: BCM305
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL16699 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version)
Samples (8)
GSM2711186 PA_1
GSM2711187 PA_2
GSM2711188 PA-TE_1
Relations
BioProject PRJNA395039

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE101635_RAW.tar 49.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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