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Status |
Public on Apr 04, 2018 |
Title |
PTEN loss and expression of the TMPRSS2/ERG fusion gene transform prostatic epithelial cells via enhanced FGF signaling |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To determine if ERG expression and PTEN loss were sufficient to transform prostate epithelial cells we constructed cell lines with expression of the TMPRSS2/ERG fusion gene (TE), stable knockdown of PTEN with shRNA (PTEN KD) or both alterations (PTEN KD/TE) using the PNT1A cell line. To determine what gene expression changes are associated the phenotypic changes between the cell line experimental groups, we carried expression microarray studies using Agilent 60K expression microarrays. RNAs from all four cell lines were analyzed in duplicate and probes with ≥ 1.4-fold or ≤0.7-fold relative to control cells identified. Our results demonstrated that PTEN loss and expression of the TMPRSS2/ERG fusion gene transform prostatic epithelial cells via enhanced FGF signaling.
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Overall design |
Microarray analyses of four cell lines (PA, PA-TE, PA-PTEN KD and PA-TE+PTEN KD).
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Contributor(s) |
Shao L, Wang J, Zhang Y, Creighton C, Ittmann M |
Citation(s) |
29581856 |
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Submission date |
Jul 19, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Chad Creighton |
E-mail(s) |
creighto@bcm.tmc.edu
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Organization name |
Baylor College of Medicine
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Department |
Biostatistics, Ducan Cancer Center
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Street address |
One Baylor Plaza, Mail Stop: BCM305
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
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Samples (8)
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Relations |
BioProject |
PRJNA395039 |