|
| Status |
Public on Jul 27, 2017 |
| Title |
Metabolic Stress Induces Modifications in the Epigenetic Program of Preimplantation Bovine Embryos |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by array
|
| Summary |
we induced an energetic stress on in vitro embryos to observe the epigenetic response associated with metabolic stress. Embryos were created with slaughterhouse cows oocytes and then exposed to an elevated glucose concentration (5mM vs 0.2mM for the control embryos) for the first 3 days post fecundation and then cultivated in normal media until blastocyst stage. Blastocysts were collected from 4 different weeks of culture. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.
|
| |
|
| Overall design |
Two condition experiment, control at 0.2 uM glucose and treatment at 5 uM glucose. 4 pools of 5 blastocysts
|
| |
|
| Contributor(s) |
Tremblay R, Dufort I, Sirard M |
| Citation(s) |
29240275 |
| Submission date |
Jul 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Marc-André Sirard |
| E-mail(s) |
Marc-Andre.Sirard@fsaa.ulaval.ca
|
| Organization name |
Université Laval
|
| Department |
Sciences Animales
|
| Street address |
Offfice 2732, 2440 Hochelaga Blvd.
|
| City |
Québec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL18384 |
EmbryoGENE DNA Methylation Array Bovine 2x400K v2.0 |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA395860 |
Less...
More...