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Series GSE101906 Query DataSets for GSE101906
Status Public on Feb 12, 2019
Title High-fat diet exposure, regardless of the induction of obesity, is associated with altered expression of genes critical for normal ovarian function
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary High-fat diet (HFD) induces diminished ovarian reserve and subfertility in female mice, irrespective of obesity. To further assess the impact of HFD on ovarian function, female mice were fed a 60% HFD or standard chow for 10 weeks and then HFD fed mice were separated into obese (HFOB) and lean (HFLN) groups. RNA-sequencing of whole ovaries identified multiple differentially expressed genes involved in normal ovarian function (ovulation, luteinization, luteolysis) in both the HFLN and HFOB mice compared to the chow fed mice (q<0.05). Taken together, elevated dietary fat intake, regardless of obesity, is associated with impaired estrous cycles and altered expression of genes critical to normal ovulatory function.
Overall design RNA-sequencing was performed on ovarian RNA after 10 weeks of HFD feeding. Three groups were assessed, those that became obese after HFD (HFOB), those that remained lean after HFD (HFLN) and controls fed chow for 10 weeks. Three animals in each group were used for RNA-sequencing.
Contributor(s) Skaznik-Wikiel ME, Hohos NM
Citation(s) 29097167
Submission date Jul 26, 2017
Last update date May 15, 2019
Contact name Malgorzata Skaznik-Wikiel
Organization name University of Colorado School of Medicine
Department Obstetrics and Gynecology
Street address 12700 East 19th Avenue
City Aurora
State/province Colorado
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (9)
GSM2718425 Control 1
GSM2718426 Control 2
GSM2718427 Control 3
BioProject PRJNA395902
SRA SRP113611

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