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Series GSE101963 Query DataSets for GSE101963
Status Public on Jul 28, 2017
Title The prominent transcriptional changes of Mycobacterium bovis BCG str. Tokyo 172 following induction by vitamin B1
Organism Mycobacterium tuberculosis variant bovis
Experiment type Expression profiling by high throughput sequencing
Summary Vitamin B1 (VB1) is a key dietary nutrient and crucial cofactor, which exhibits a series of regulatory functions on cellular processes and the activation of the immune system. To date, the precise effect of VB1 on Mycobacterium tuberculosis has not been fully described. In the present study, the direct influence of VB1 treatment on M. bovis BCG was determined using RNA-sequencing. The selection of this strain was used due to its common physiological features with M.tuberculosis. The investigation of the M. bovis BCG transcription demonstrated significant changes in certain metabolic and cellular process such as the decrease in fatty acid, cholesterol and glycolipid catabolism, the decrease in DNA replication and protein translation, the reduction in cell division and cell wall formation and the induction of arginine biosynthesis. In addition, growth assays indicated that VB1 inhibited the BCG growth rate in vitro, whereas LC/MS analysis demonstrated that the concentration of arginine was higher following VB1 supplementation. It is suggested that VB1 could be used for the treatment of tuberculosis potentially.
 
Overall design 2 samples
 
Contributor(s) Song N, Li Z, Cui Z, Chen L, Cui Y, Dang G, Li Z, Li H, Liu S
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Submission date Jul 27, 2017
Last update date May 15, 2019
Contact name Ningning Song
E-mail(s) songning19810508@hotmail.com
Phone 0451-50151732
Organization name Harbin Veterinary Research Institute
Street address No.678. Haping Road
City harbin
ZIP/Postal code 150069
Country China
 
Platforms (1)
GPL23838 Illumina HiSeq 2500 (Mycobacterium bovis)
Samples (2)
GSM2719686 AA
GSM2719687 VB1
Relations
BioProject PRJNA396028
SRA SRP113698

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE101963_processed_data.xls.gz 175.6 Kb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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