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| Status |
Public on Oct 07, 2017 |
| Title |
Single cell RNA Seq data of BMDM from mouse infected with Salmonella [stat log] |
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The interaction between a pathogen and a host is a highly dynamic process in which both agents activate complex programs. Here, we introduce a single-cell RNA-Seq method (scDual-Seq) that simultaneously captures both host and pathogen transcriptomes and use it to study the process of infection of individual mouse macrophages with the intracellular pathogen Salmonella typhimurium. Among the infected macrophages, we found three subpopulations and we show evidence for a linear progression through these subpopulations, supporting a model in which these three states correspond to consecutive stages of infection.
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| |
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| Overall design |
salmonella RNA extracted from liquid stationary culture
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| |
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| Contributor(s) |
Avital G, Avrahanm R, Fan A, Hashimshony T, Hung D, Yanai I |
| Citation(s) |
29073931 |
| |
| Submission date |
Aug 02, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Itai Yanai |
| E-mail(s) |
Itai.Yanai@nyumc.org
|
| Organization name |
New York University, School of Medicine
|
| Department |
Biochemistry and Molecular Pharmacology
|
| Lab |
Itai Yanai
|
| Street address |
430 East 29th St., Office 510
|
| City |
New York |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19729 |
Illumina HiSeq 2500 (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE102163 |
Single cell RNA Seq data of BMDM from mouse infected with Salmonella |
|
| Relations |
| BioProject |
PRJNA396894 |
| SRA |
SRP114680 |
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