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Series GSE102324 Query DataSets for GSE102324
Status Public on Jul 31, 2018
Title Overexpression of sigD in the wild type
Platform organism Corynebacterium glutamicum
Sample organism Corynebacterium glutamicum R
Experiment type Expression profiling by array
Summary (Coryno)mycolate is a α-branched, β-hydroxylated long-chain fatty acid specifically synthesized in bacteria in the suborder Corynebacterineae of the phylum Actinobacteria. It forms an outer membrane and functions as a permeability barrier conferring pathogenic mycobacteria to resistance to antibiotics. Whereas mycolate biosynthetic pathway has been intensively studied, the studies on the transcriptional regulation of genes involved in the pathway are limited. Here, we report that the previously uncharacterized extracytoplasmic function σ factor, σD, is a key regulator of the mycolate synthetic genes in Corynebacterium glutamicum in the suborder. Chromatin immunoprecipitation with microarray analysis detected σD-binding regions in the genome, establishing a consensus promoter sequence for σD recognition. The σD regulon comprises acyl-CoA carboxylase subunits, acyl-AMP ligase, polyketide synthase, and mycolyltransferases, all of which are involved in mycolate synthesis. Actually, deletion or overexpression of sigD encoding σD modified the amount of extractable mycolate. Immediately downstream of sigD, rsdA encoded anti-σD and was under the control of a σD-dependent promoter. Another σD regulon member, L,D-transpeptidase, conferred lysozyme resistance. Thus, σD modifies cell wall composition and enhances mycolate synthesis to provide resistance to environmental stress.
 
Overall design Comparison of gene expression profiles of the wild type before and after sigD overexpression at the exponential phase. Four independent experiments were performed.
 
Contributor(s) Toyoda K, Inui M
Citation(s) 29148103
Submission date Aug 07, 2017
Last update date Jul 25, 2021
Contact name Masayuki Inui
E-mail(s) mmg-lab@rite.or.jp
Organization name Research institute of Innovative Technology for the Earth (RITE)
Street address 9-2 Kizugawadai, Kizugawa
City Kyoto
ZIP/Postal code 619-0292
Country Japan
 
Platforms (1)
GPL20864 Corynebacterium glutamicum CGR_Oligo_array
Samples (7)
GSM2734733 sigD_overexpress_1st
GSM2734734 sigD_overexpress_2nd
GSM2734735 sigD_overexpress_3rd
This SubSeries is part of SuperSeries:
GSE102329 Genome-wide analyses of C. glutamicum SigD
Relations
BioProject PRJNA397507

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102324_RAW.tar 4.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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