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| Status |
Public on Jul 31, 2018 |
| Title |
Overexpression of sigD in the wild type |
| Platform organism |
Corynebacterium glutamicum |
| Sample organism |
Corynebacterium glutamicum R |
| Experiment type |
Expression profiling by array
|
| Summary |
(Coryno)mycolate is a α-branched, β-hydroxylated long-chain fatty acid specifically synthesized in bacteria in the suborder Corynebacterineae of the phylum Actinobacteria. It forms an outer membrane and functions as a permeability barrier conferring pathogenic mycobacteria to resistance to antibiotics. Whereas mycolate biosynthetic pathway has been intensively studied, the studies on the transcriptional regulation of genes involved in the pathway are limited. Here, we report that the previously uncharacterized extracytoplasmic function σ factor, σD, is a key regulator of the mycolate synthetic genes in Corynebacterium glutamicum in the suborder. Chromatin immunoprecipitation with microarray analysis detected σD-binding regions in the genome, establishing a consensus promoter sequence for σD recognition. The σD regulon comprises acyl-CoA carboxylase subunits, acyl-AMP ligase, polyketide synthase, and mycolyltransferases, all of which are involved in mycolate synthesis. Actually, deletion or overexpression of sigD encoding σD modified the amount of extractable mycolate. Immediately downstream of sigD, rsdA encoded anti-σD and was under the control of a σD-dependent promoter. Another σD regulon member, L,D-transpeptidase, conferred lysozyme resistance. Thus, σD modifies cell wall composition and enhances mycolate synthesis to provide resistance to environmental stress.
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| Overall design |
Comparison of gene expression profiles of the wild type before and after sigD overexpression at the exponential phase. Four independent experiments were performed.
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| Contributor(s) |
Toyoda K, Inui M |
| Citation(s) |
29148103 |
| |
| Submission date |
Aug 07, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Masayuki Inui |
| E-mail(s) |
mmg-lab@rite.or.jp
|
| Organization name |
Research institute of Innovative Technology for the Earth (RITE)
|
| Street address |
9-2 Kizugawadai, Kizugawa
|
| City |
Kyoto |
| ZIP/Postal code |
619-0292 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL20864 |
Corynebacterium glutamicum CGR_Oligo_array |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE102329 |
Genome-wide analyses of C. glutamicum SigD |
|
| Relations |
| BioProject |
PRJNA397507 |
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