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| Status |
Public on Feb 19, 2018 |
| Title |
Response of the Drosophila Schneider cells to the 20-hydroxyecdysone |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The subject of the current study was the response of the Drosophila Schneider cells to the developmental hormone 20-hydroxyecdysone. The whole-genome experiments revealed that ecdysone-dependent genes are at high level of readiness to the upcoming transcription. Their promoters in the inactive state are associated with significant amounts of the RNA polymerase II. The induction of transcription by 20-hydroxyecdysone results both in the recruitment of the additional portion of the RNA polymerase II and in the increase in its phosphorylation level. The primary events in the course of the transcriptional activation of the two ecdysone-dependent genes have been investigated to detail.
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| Overall design |
ChIP-Seq of RNA polymerase II states (total, Ser2Phospho and Ser5Phospho), coregulators (Brm, KisL, CHD1, dSet1, lid/Kdm5, Gcn5, DART1, Spt5, cdk8 and PAF1) and ChIP H3-Mnase assay (with antibodies against Histone H3 and Histone H3K27Ac) were performed in S2 Scheider cell after 20-hydroxyecdysone treatment and in sham-treated cells (DMSO-treated)
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| Contributor(s) |
Vorobyeva NE, Krasnov AN |
| Citation(s) |
29410380 |
| |
| Submission date |
Aug 10, 2017 |
| Last update date |
Jun 13, 2023 |
| Contact name |
Nadezhda E Vorobyeva |
| E-mail(s) |
nvorobyova@gmail.com
|
| Phone |
+79262790219
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| Organization name |
Institute of Gene Biology RAS
|
| Street address |
Vavilova 34/5
|
| City |
Moscow |
| ZIP/Postal code |
119334 |
| Country |
Russia |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA397928 |
| SRA |
SRP115238 |
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