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| Status |
Public on Mar 18, 2021 |
| Title |
Elevated Expression of Two Circulating LncRNAs Predicts Poor Prognosis for Acute Cerebral Infarction |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
Expression profiling by array
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| Summary |
Poor prognosis for acute cerebral infarction (ACI) was suggested to be predicted using the high expression of some novel molecular markers, for example long non-coding RNAs (lncRNAs). Differentially expressed lncRNAs in peripheral whole blood from the ACI patients and healthy volunteers(HVT) were identified using microarray and further verified by qRT-PCR. The clinical outcome evaluated by 3-month modified Rankin Score (mRS), stroke stratification classified by OCSP criteria, and the expression characteristics of specific lncRNAs were analyzed in ACI patients. Among 5686 differentially expressed lncRNAs screened out by the microarray, nine were verified by qRT-PCR. Particularly, the expression of NR_120420 and lnc-GCH1-2:3 in the ACI group were significantly higher than the HVT group. ROC analysis showed that the sensitivity and specificity of NR_120420 were 85.7% and 84.6% in patients with total anterior circulation infarction (TACI) respectively(area under curveļ¼AUC=0.861), while the sensitivity and specificity of lnc-GCH1-2:3 were 85.7% and 82.1% in patients with TACI respectively(AUC=0.802). Multivariate logistic regression showed that NR_120420 was a significantly independent diagnostic factor for ACI. The elevated expression levels of NR_120420 and lnc-GCH1-2:3 were associated with the TACI stroke classification and the poor prognosis of ACI. Our study clearly illustrated that the elevated expression levels of circulating NR_120420 and lnc-GCH1-2:3 could predict the TACI stroke classification with high sensitivity and specificity and the poor prognosis of patients with ACI.
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| Overall design |
Peripheral whole blood from patients with acute cerebral infarction (ACI) (n=6) and healthy controls (HVT) (n=3) were screened by microarray to find differentially expressed lncRNAs.Time duration from stroke onset to admission was less than 6 hours.
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| Contributor(s) |
Tian C, Hong B |
| Citation(s) |
35251465 |
| |
| Submission date |
Aug 11, 2017 |
| Last update date |
Mar 10, 2022 |
| Contact name |
tian chunou |
| E-mail(s) |
tianchunou@126.com
|
| Organization name |
Changhai Hospital, Second Military Medical University, China
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| Department |
Neurosurgery Department
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| Street address |
168 changhai road
|
| City |
shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL22755 |
Agilent-076500 Human lncRNA+mRNA array (Probe name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA397986 |
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