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| Status |
Public on Aug 10, 2018 |
| Title |
Queuosine modification protects cognate tRNAs against ribonuclease cleavage |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Eukaryotic transfer RNAs (tRNA) contain on average 13 modifications that perform a wide range of roles in translation and in the generation of tRNA fragments that regulate gene expression. Queuosine (Q) modification occurs in the wobble anticodon position of tRNAs for amino acids His, Asn, Tyr, and Asp. In eukaryotes, Q modification is fully dependent on diet or on gut microbiome in multi-cellular organisms. Despite decades of study, cellular roles of Q modification remain to be fully elucidated. Here we show that in human cells, Q modification specifically protects its cognate tRNAHis and tRNAAsn against cleavage by ribonucleases. We generated cell lines that contain completely depleted or fully Q-modified tRNAs. Using these resources, we found that Q modification significantly reduces angiogenin cleavage of its cognate tRNAs in vitro. Q modification does not change the cellular abundance of the cognate full-length tRNAs, but alters the cellular content of their fragments in vivo in the absence and presence of stress. Our results provide a new biological aspect of Q modification and a mechanism of how Q modification alters small RNA pool in human cells.
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| Overall design |
Comprehensive comparative analysis of high throughput sequencing data to determine queuosine-mediated tRNA fragments
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| Contributor(s) |
Wang X, Matuszek Z, Huang Y, Parisien M, Clark W, Schwartz MH, Dai Q, Pan T |
| Citation(s) |
29970597 |
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| Submission date |
Aug 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Tao Pan |
| E-mail(s) |
taopan@uchicago.edu
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| Phone |
(773) 702-4179
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| Organization name |
University of Chicago
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| Department |
Biochemistry and Molecular Biology
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| Street address |
929 E. 57th Street
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| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
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| Samples (4)
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| GSM2741321 |
HEK293T AlkB untreated, Queuosine Unsupplemented |
| GSM2741322 |
HEK293T AlkB treated, Queuosine Unsupplemented |
| GSM2741323 |
HEK293T AlkB untreated, Queuosine Supplemented |
| GSM2741324 |
HEK293T AlkB treated, Queuosine Supplemented |
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| Relations |
| BioProject |
PRJNA398048 |
| SRA |
SRP115314 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE102570_MP_fragment_analysis.xlsx.gz |
1.0 Mb |
(ftp)(http) |
XLSX |
| GSE102570_RAW.tar |
11.8 Mb |
(http)(custom) |
TAR (of TXT, ZIP) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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