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| Status |
Public on Aug 26, 2018 |
| Title |
Depletion of ZNF185 causes delay of keratinocyte differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Development of epidermis includes a complicated program of keratinocyte differentiation. Here we study a new membrane LIM-domain containing Zn-finger protein ZNF185 which is expressed in upper layers of human skin and is up-regulated during keratinocyte differentiation in vitro. Interestingly, depletion of ZNF185 causes delay of keratinocyte differentiation with decreased levels of FLG, LOR, LCEs expression.
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| Overall design |
To study the role of ZNF185 in keratinocyte differentiation, we performed microarray expression profiling to identify the genes which expression is altered. Human normal epidermal keratinocytes (HEKn) we transiently knocked-down by RNA interference and induced for differentiation for 3 days.
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| Contributor(s) |
Smirnov A |
| Citation(s) |
30337687 |
| |
| Submission date |
Aug 14, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Artem Smirnov |
| E-mail(s) |
art.smirnow@gmail.com
|
| Organization name |
University of Rome "Tor Vergata"
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| Department |
Dept. of Exp. Medicine and Surgery
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| Lab |
Molecular biology lab (Prof. G. Melino)
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| Street address |
Via Montpellier, 1
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| City |
Rome |
| ZIP/Postal code |
00133 |
| Country |
Italy |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA398210 |
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