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Series GSE102695 Query DataSets for GSE102695
Status Public on Jan 16, 2018
Title Comparative profiling of the heterochromatin landscape in different life cycle stages, strains and species of malaria parasites
Organisms Plasmodium berghei; Plasmodium chabaudi; Plasmodium falciparum; Plasmodium knowlesi; Plasmodium vivax; Plasmodium yoelii
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Heterochromatin is a tightly packaged form of DNA that leads to permanent or temporal gene silencing. In P. falciparum heterochromatin is formed after trimethylation of lysine 9 on histone H3 and consequent binding of heterochromatin protein 1 (HP1). Genome-wide profiling studies established that in P. falciparum blood stage schizonts heterochromatin is formed at subtelomeres and some intra-chromosomal islands. Reversible silencing of genes located in these regions has been shown to be key to, among others, antigenic variation, invasion pathway selection or commitment to gametocytogenesis. However, heterochromatic gene silencing has not been investigated in many other Plasmodium species, strains or life cycle stages, yet. Here, we used ChIP-seq to profile HP1 occupancy in asexual parasites of six different species (P. falciparum, P. knowlesi, P. vivax, P. berghei, P. yoelii, and P. chabaudi) and of four different P. falciparum strains (3D7, PF2004, NF54, NF135) as well as two different P. knowlesi clones (A1C1, A1H1). Additionally, we performed HP1 ChIP-seq on three asexual stages (ring, trophozoite and schizont stage) and two sexual stages of P. falciparum parasites. Collectively, the generated HP1 profiles provide a detailed catalog of heterochromatic genes and reveal conserved and specialized features of epigenetic control at different life cycle stages, strains and species across the genus Plasmodium.
 
Overall design In total, we sequenced 26 samples (14 HP1-ChIP-seq samples and 12 inputs) generated from chromatin from six different Plasmodium species (P. chabaudi, P. berghei, P. yoelii, P. knowlesi, P. vivax and P. falciparum), four different strains of P. falciparum (3D7, NF54, NF135 and Pf2004) and two different strains of P. knowlesi (A1.C1 and A1.H1) as well as three different intraerythrocytic stages of the P. falciparum 3D7 strain (ring, trophozoite and schizont stages) and three different stages of the P. falciparum Pf2004 strain (schizont stages, stage II/III gametocytes and stage IV/V gametocytes).
 
Contributor(s) Fraschka SA, Filarsky M, Hoo R, Niederwieser I, Yan Yam X, Brancucci NB, Mohring F, Mushunje AT, Huang X, Christensen PR, Nosten F, Russell B, Moon RW, Marti M, Preiser PR, Bartfai R, Voss TS
Citation(s) 29503181
Submission date Aug 15, 2017
Last update date May 15, 2019
Contact name Sabine Fraschka
Organization name Radboud University Nijmegen
Department Molecular Biology
Lab Richárd Bártfai
Street address Geert Grooteplein 28
City Nijmegen
ZIP/Postal code 6525 GA
Country Netherlands
 
Platforms (6)
GPL21298 Illumina NextSeq 500 (Plasmodium falciparum)
GPL23133 Illumina NextSeq 500 (Plasmodium berghei)
GPL23913 Illumina NextSeq 500 (Plasmodium chabaudi)
Samples (26)
GSM2743112 Pf-3D7-Ring-HP1
GSM2743113 Pf-3D7-Trophozoite-HP1
GSM2743114 Pf-3D7-Schizont-HP1
Relations
BioProject PRJNA398396
SRA SRP115493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102695_P.berghei-HP1_over_P.berghei-Input.Bedgraph.gz 125.5 Mb (ftp)(http) BEDGRAPH
GSE102695_P.chabaudi-HP1_over_P.chabaudi-Input.Bedgraph.gz 140.1 Mb (ftp)(http) BEDGRAPH
GSE102695_P.knowlesi-A1C1-HP1_over_P.knowlesi-A1C1-Input.Bedgraph.gz 160.7 Mb (ftp)(http) BEDGRAPH
GSE102695_P.knowlesi-A1H1-HP1_over_P.knowlesi-A1H1-Input.Bedgraph.gz 160.6 Mb (ftp)(http) BEDGRAPH
GSE102695_P.vivax-HP1_over_P.vivax-Input.Bedgraph.gz 123.8 Mb (ftp)(http) BEDGRAPH
GSE102695_P.yoelii-HP1_over_P.yoelii-Input.Bedgraph.gz 143.9 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-3D7-Ring-HP1_over_Pf-3D7-Schizont-Input.Bedgraph.gz 124.7 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-3D7-Schizont-HP1_over_Pf-3D7-Schizont-Input.Bedgraph.gz 116.5 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-3D7-Trophozoite-HP1_over_Pf-3D7-Schizont-Input.Bedgraph.gz 109.8 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-NF135-Schizont-HP1_over_Pf-NF135-Schizont-Input.Bedgraph.gz 107.8 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-NF54-Schizont-HP1_over_Pf-NF54-Schizont-Input.Bedgraph.gz 118.0 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-Pf2004-GamII-III-HP1_over_Pf-Pf2004-GamII-III-Input.Bedgraph.gz 86.7 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-Pf2004-GamII-III-HP1_over_Pf-Pf2004-GamII-III-Input_Pf2004-assembly.Bedgraph.gz 92.9 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-Pf2004-GamIV-V-HP1_over_Pf-Pf2004-GamIV-V-Input.Bedgraph.gz 124.1 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-Pf2004-GamIV-V-HP1_over_Pf-Pf2004-GamIV-V-Input_Pf2004-assembly.Bedgraph.gz 129.0 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-Pf2004-Schizont-HP1_over_Pf-Pf2004-Schizont-Input.Bedgraph.gz 92.4 Mb (ftp)(http) BEDGRAPH
GSE102695_Pf-Pf2004-Schizont-HP1_over_Pf-Pf2004-Schizont-Input_Pf2004-assembly.Bedgraph.gz 98.8 Mb (ftp)(http) BEDGRAPH
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