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| Status |
Public on Jan 01, 2018 |
| Title |
Gene expression profiling of the Tlr2 mutant of zebrafish embryos at 4 days post infection of M. marinum |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We use the Tlr2 mutant of zebrafish embryos model to study the transcriptome response to Mycobacterium marinum infection. We injected M.marinum into the caudal vein at 28 hours post fertilization and took samples at 4 days post infection.
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| Overall design |
This deep sequence study was designed to determine the gene expression profile in the Tlr2 mutant and heterozygote by M.marinum infection. RNA was isolated at 4 days post infection. Tlr2 mutants and heterozygotes zebrafish embryos were micro-injected into the caudal vein with 150CFU M.marinum, or PBS as a control at 28 hours post fertilization. After injections embryso were transerred into fresh egg water and incubated at 28 degree. At 4 days post infection triplicateds of 10 embryos per condition were snapfrozen in liquid nitogen, and total RNA was isolated using TRIZOL reagent.
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| Contributor(s) |
Yang S, Spaink H |
| Citation(s) |
31747871 |
| |
| Submission date |
Aug 17, 2017 |
| Last update date |
Dec 17, 2019 |
| Contact name |
Herman Pieter Spaink |
| E-mail(s) |
h.p.spaink@biology.leidenuniv.nl
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| Phone |
31715275055
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| Organization name |
Leiden University
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| Department |
Institute of Biology
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| Lab |
Molecular Cell Biology
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| Street address |
Einsteinweg 55
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| City |
Leiden |
| ZIP/Postal code |
2333 CC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA398644 |
| SRA |
SRP115629 |
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