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Series GSE102823 Query DataSets for GSE102823
Status Public on Feb 11, 2019
Title Gene expression profiling of CAR-T cells pre-and post-adoptive transfer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Chimeric Antigen Receptors (CARs) are synthetic immune receptors introduced in cancer patients' autologous T Cells for cellular immunotherapy. The purpose of this study is to characterize the changes in gene expression induced by the presence of a CAR in human primary T cells (comparison A or B versus C); and the changes that take place for each group after transfer into a xenograft model of pancreatic cancer (comparison Post versus Pre).
 
Overall design Primary human CD8 T cells were stimulated ex vivo and transduced to express either of 2 variants of an anti-PSCA CAR, or GFP as a control. Three independent cultures were maintained as biological replicates for each group. Two weeks later, they were injected into immunodeficient mice bearing HPAC-derived pancreatic tumors. Spleen-resident human CD8 T cells were isolated 30 days later, and the gene expression profiles of each group was analyzed using microarrays.
 
Contributor(s) Abate-Daga D, Yoder S
Citation(s) 30755478
Submission date Aug 18, 2017
Last update date Jul 25, 2021
Contact name Daniel Abate-Daga
E-mail(s) daniel.abatedaga@moffitt.org
Phone 813-745-6856
Organization name H. Lee Moffitt Cancer Center and Research Institute
Department Immunology
Street address 12902 Magnolia Dr.
City Tampa
State/province Florida
ZIP/Postal code 33612
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (18)
GSM2746860 PSCA3_post, biological replicate1
GSM2746861 PSCA3_post, biological replicate2
GSM2746862 PSCA3_post, biological replicate3
Relations
BioProject PRJNA399087

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102823_RAW.tar 87.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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