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GEO help: Mouse over screen elements for information. |
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Status |
Public on Feb 11, 2019 |
Title |
Gene expression profiling of CAR-T cells pre-and post-adoptive transfer |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Chimeric Antigen Receptors (CARs) are synthetic immune receptors introduced in cancer patients' autologous T Cells for cellular immunotherapy. The purpose of this study is to characterize the changes in gene expression induced by the presence of a CAR in human primary T cells (comparison A or B versus C); and the changes that take place for each group after transfer into a xenograft model of pancreatic cancer (comparison Post versus Pre).
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Overall design |
Primary human CD8 T cells were stimulated ex vivo and transduced to express either of 2 variants of an anti-PSCA CAR, or GFP as a control. Three independent cultures were maintained as biological replicates for each group. Two weeks later, they were injected into immunodeficient mice bearing HPAC-derived pancreatic tumors. Spleen-resident human CD8 T cells were isolated 30 days later, and the gene expression profiles of each group was analyzed using microarrays.
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Contributor(s) |
Abate-Daga D, Yoder S |
Citation(s) |
30755478 |
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Submission date |
Aug 18, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Daniel Abate-Daga |
E-mail(s) |
daniel.abatedaga@moffitt.org
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Phone |
813-745-6856
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Organization name |
H. Lee Moffitt Cancer Center and Research Institute
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Department |
Immunology
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Street address |
12902 Magnolia Dr.
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City |
Tampa |
State/province |
Florida |
ZIP/Postal code |
33612 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (18)
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GSM2746863 |
PSCA3_pre, biological replicate1 |
GSM2746864 |
PSCA3_pre, biological replicate2 |
GSM2746865 |
PSCA3_pre, biological replicate3 |
GSM2746866 |
PSCA2_post, biological replicate1 |
GSM2746867 |
PSCA2_post, biological replicate2 |
GSM2746868 |
PSCA2_post, biological replicate3 |
GSM2746869 |
PSCA2_pre, biological replicate1 |
GSM2746870 |
PSCA2_pre, biological replicate2 |
GSM2746871 |
PSCA2_pre, biological replicate3 |
GSM2746872 |
GFP_post, biological replicate1 |
GSM2746873 |
GFP_post, biological replicate2 |
GSM2746874 |
GFP_post, biological replicate3 |
GSM2746875 |
GFP_pre, biological replicate1 |
GSM2746876 |
GFP_pre, biological replicate2 |
GSM2746877 |
GFP_pre, biological replicate3 |
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Relations |
BioProject |
PRJNA399087 |
Supplementary file |
Size |
Download |
File type/resource |
GSE102823_RAW.tar |
87.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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