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| Status |
Public on Aug 26, 2017 |
| Title |
Differential expression profile of mRNAs and lncRNAs regulated by HNF1α in hepatocellular carcinoma cells HuH7 |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
Expression profiling by array
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| Summary |
To determine the role of HNF1α on long noncoding RNAs (lncRNAs) and the overall mechanisms in hepatocellular carcinoma, we analyzed the expression profiles of lncRNAs and mRNAs in huh7 cells with overexpression or knockdown of HNF1α. Expression profile showed that there were 339 mRNAs and 22 intergenic lncRNAs positively regulated by HNF1α for more than 2 times. The up-regulated genes separated samples by comparing lenti-HNF1α group with lenti-ctrl group, with overlap the down-regulated genes separated in HNF1α knockdown groups were identified as the genes positived regulated by HNF1α. Expression of seven genes from these 22 intergenic lncRNAs was quantified in the same RNA samples by real-time PCR, confirming the regulatory effect of HNF1α on lncRNAs as well as the microarray analysis pattern.
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| Overall design |
HuH7 cells were infected with lentivirus which carrying HNF1α or shHNF1α with their control group, respectively. The total RNA of four groups (lenti-ctrl, lenti-HNF1α, lenti-shNC, lenti-shHNF1α) was measured by microarray at 6 days after HuH7 cells infected with lentivirus. And then we analyzed the mRNAs expression profile and lncRNAs expression profile, respectively.
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| Contributor(s) |
Ding C, Zhang X, Xie W |
| Citation(s) |
29466992 |
| Submission date |
Aug 25, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
xin zhang |
| E-mail(s) |
zhang68@hotmail.com
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| Organization name |
Changzheng hospital
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| Street address |
415 Fengyang Road
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| City |
Shanghai |
| ZIP/Postal code |
200003 |
| Country |
China |
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| Platforms (1) |
| GPL18180 |
Agilent-045142 Human LncRNA v4 4X180K [Probe Name Version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA400249 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103128_RAW.tar |
101.2 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE103128_nor_data.txt.gz |
4.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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