|
| Status |
Public on Sep 01, 2017 |
| Title |
Transcriptome profiling of individual rhesus macaque oocytes and preimplantation embryos |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Early mammalian embryonic transcriptomes are dynamic throughout the process of preimplantation development. Cataloging of primate transcriptomics during early development has been accomplished in humans, but global characterization of transcripts is lacking in the rhesus macaque: a key model for human reproductive processes. We report here the systematic classification of individual macaque transcriptomes using RNA-Seq technology from the germinal vesicle stage oocyte through the blastocyst stage embryo. Major differences in gene expression were found between sequential stages, with the 4- to 8-cell stages showing the highest level of differential gene expression. Analysis of putative transcription factor binding sites also revealed a striking increase in key regulatory factors in 8-cell embryos, indicating a strong likelihood of embryonic genome activation occurring at this stage. Furthermore, clustering analyses of gene co-expression throughout this period resulted in distinct groups of transcripts significantly associated to the different embryo stages assayed. The sequence data provided here along with characterizations of major regulatory transcript groups presents a comprehensive atlas of polyadenylated transcripts that serves as a useful resource for comparative studies of preimplantation development in humans and other species.
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| |
|
| Overall design |
RNA-seq analysis in single oocytes/embryos at different stages of development including 2-3 replicates per stage.
|
| |
|
| Contributor(s) |
Ross P, Chitwood J |
| Citation(s) |
29025079 |
| |
| Submission date |
Aug 30, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Pablo Juan Ross |
| E-mail(s) |
pross@ucdavis.edu
|
| Phone |
5307717225
|
| Organization name |
University of California Davis
|
| Department |
Animal Science
|
| Lab |
Ross
|
| Street address |
1 Shields Ave, 2239 Meyer Hall
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14954 |
Illumina HiSeq 2000 (Macaca mulatta) |
|
| Samples (22)
|
|
| Relations |
| BioProject |
PRJNA401876 |
| SRA |
SRP122953 |
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