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Series GSE10335 Query DataSets for GSE10335
Status Public on Dec 31, 2020
Title Transcriptional profiling of shoot of OsPI1 knockdown rice
Platform organism Oryza sativa Japonica Group
Sample organism Oryza sativa
Experiment type Expression profiling by array
Summary OsPI1 was isolated from rice and revealed to belong to the TPSI1/Mt4 family, a phosphorus deficiency responsive gene family. The importance of this family gene for phosphorus deficiency responses of plants had been already reported, though its actual role is still unclear. To understand the plants response mechanisms for phosphorus deficiency and the role of OsPI1 in it, we constructed a OsPI1 RNAi line (ospi1-2) for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the shoots and roots of ospi1-2 and Oryza sativa L. cv. Kitaake wild type (WT) rice plants under constitutive and phosphorus deficiency at seedling stage.
Keywords: Influences of OsPI1 knockdown
 
Overall design We used Agilent rice gene chips (G4138A) to investigate the transcript level changes in rice plant tissues during phosphorus deficiency. We used an OsPI1 knockdown line (ospi1-2) and wild type (WT). Plants were grown in growth chambers and treated in nutrient solutions with or without 32 μM NaH2PO4 for 9 d. Three plants were harvested for one replication and each sample was divided into roots and shoots. Two replications of microarray experiments using two biological replications were carried out by hybridizing the RNA from ospi1-2 and WT plants on the same slide.
 
Contributor(s) Nishiyama T, Wasaki J, Choi S, Kuroda S, Ito S, Shinano T, Osaki M
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Submission date Jan 31, 2008
Last update date Jan 01, 2021
Contact name Jun Wasaki
E-mail(s) junw@hiroshima-u.ac.jp
Organization name Hiroshima University
Department Graduate School of Biosphere Science
Street address Kagamiyama 1-7-1
City Higashi-Hiroshima
State/province Hiroshima
ZIP/Postal code 739-8521
Country Japan
 
Platforms (1)
GPL892 Agilent-012106 Rice Oligo Microarray G4138A (Feature Number version)
Samples (8)
GSM261333 shoot of OsPI1 knockdown rice grown under phosphorus deficiency, replication 1.
GSM261334 shoot of OsPI1 knockdown rice grown under phosphorus deficiency, replication 2.
GSM261335 root of OsPI1 knockdown rice grown under phosphorus deficiency, replication 1.
Relations
BioProject PRJNA105571

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10335_RAW.tar 44.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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