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| Status |
Public on Feb 01, 2024 |
| Title |
Genome-wide binding profile of Halo-tagged cohesin |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We determined the genome-wide binding profile of CTCF and cohesin subunits Smc1a and Smc3 in wild type mouse ES cells and compared them to a double FLAG-Halo-mCTCF/mRad21-SNAPf-V5 knock-in mES line, generated by CRISPR/Cas9-mediated genome editing to perform imaging experiments.
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| Overall design |
ChIP-Seq of CTCF, Smc1a and Smc3 cohesin subunits in wild type mouse ES cells (WT) and in mouse ES cells homozygous for FLAG-Halo-CTCF and Rad21-SNAPf-V5 (C59 clone). ChIP-Seq libraries were prepared using normal serum IgGs as a control, and including total inputs as reference datasets.
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| Contributor(s) |
Sejr Hansen A, Cattoglio C, Tjian R, Darzacq X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 19, 2017 |
| Last update date |
Feb 01, 2024 |
| Contact name |
Robert Tjian |
| E-mail(s) |
jmlim@berkeley.edu
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| Phone |
(510) 642-0884
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| Organization name |
UC Berkeley
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| Department |
MCB
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| Lab |
Tjian Lab
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| Street address |
University of California, Berkeley 450 Li Ka Shing #3370
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA407925 |
| SRA |
SRP118089 |
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