|
| Status |
Public on Feb 12, 2019 |
| Title |
Transcriptional Analysis of Foxp3+ Regulatory T Cells in Experimental Acute Lung Injury Resolution |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
These studies profiled the expression of mRNA in lung and spleen regulatory T cells in Foxp3EGFP mice during lipopolysaccharide-induced lung injury.
Baseline, uninjured Lung Treg and Resolving Lung Tregs and Resolving Splenic Tregs were sorted (7 days after intratracheal instillation of LPS for Resolving conditions) and mRNAs differentially expressed (DE) between Control Lung Tregs, Resolving Lung Tregs and Resolving Splenic Tregs samples were identified using microarrays.
|
| |
|
| Overall design |
Baseline, uninjured Lung Treg and Resolving Lung Tregs and Resolving Splenic Tregs were sorted for mRNA analysis. mRNA expression was profiled using Mouse Gene 2.1 ST arrays (Affymetrix, Santa Clara, CA) which cover over 28,000 RefSeq transcripts.
|
| |
|
| Contributor(s) |
Mock JR, Dang H, Doerschuk CM |
| Citation(s) |
30753170 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K08 HL129075 |
Regulatory T Cells Promote Alveolar Epithelial Repair |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Jason Robert Mock |
| R03 HL145255 |
Defining the Role of Regulatory T Cell-Derived MMP12 in Acute Lung Injury Resolution |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Jason Robert Mock |
| K12 HL119998 |
Application of Omics in Lung Disease |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Claire M Doerschuk |
|
| |
| Submission date |
Sep 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jason Robert Mock |
| E-mail(s) |
jason_mock@med.unc.edu
|
| Phone |
9199625347
|
| Organization name |
University of North Carolina
|
| Department |
Internal Medicine
|
| Street address |
125 Mason Farm Rd, Marsico Hall 7229G
|
| City |
Chapel Hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22070 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript-level na35ens81] |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA408182 |
Less...
More...