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Series GSE104211 Query DataSets for GSE104211
Status Public on Sep 30, 2017
Title Transcriptional response of bovine moncocyte-derived macrophages to infection with strains of Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis
Organism Bos taurus
Experiment type Expression profiling by high throughput sequencing
Summary Mycobacterium bovis (M. bovis) and Mycobacterium avium subspecies paratuberculosis (MAP) are important pathogens of cattle, causing bovine tuberculosis and Johne’s disease respectively. M. bovis and MAP infect residential macrophages in the lung and intestines respectively and subvert the macrophage biology to create a survival niche. To investigate this interaction we simultaneously studied the transcriptional response of bovine monocyte-derived macrophages to infection with two strains of M. bovis (AF2122/97 and G18) and two strains of MAP (C & L1).
 
Overall design 120 samples were analysed in total; derived from six biological replicates (i.e. cells isolated from six cattle). Cells were left uninfected (medium only controls) or infected with either a M. bovis strain (AF2122/97 or G18) or a MAP strain (C or L1). Cells were harvested at 2, 6, 24 and 48h post infection.
 
Contributor(s) Jensen K, Gallagher IJ, Johnston N, Welsh M, Skuce R, Williams JL, Glass EJ
Citation(s) 29263113
Submission date Sep 25, 2017
Last update date Jul 25, 2021
Contact name Iain Gallagher
E-mail(s) iaingallagher@gmail.com
Phone 00 44 1786 46 6024
Organization name University of Stirling
Department Faculty of Health Sciences and Sport
Street address Room 4B133 Cottrell Building, University of Stirling, Airthrey Road
City Stirling
ZIP/Postal code FK9 4LA
Country United Kingdom
 
Platforms (1)
GPL11153 Illumina Genome Analyzer II (Bos taurus)
Samples (80)
GSM2792498 489_017
GSM2792499 489_018
GSM2792500 489_022
Relations
BioProject PRJNA412041
SRA SRP118815

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Supplementary file Size Download File type/resource
GSE104211_RAW.tar 8.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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