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| Status |
Public on Nov 02, 2018 |
| Title |
The Peril DNA locus acts as an in vivo enhancer for distal but not proximal genes |
| Organisms |
Mus musculus; synthetic construct |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
(1) We have performed a massively parallel reporter assay (MPRA) in C2C12 cells. Additionally, we have (2) sequenced wildtype and mutant ganglionic emminence and olfactory bulb (GE/OB), and kidney from E14.5 mouse pups, as well as mESC from the mutant strain Periltm1.1Vlcg.
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| |
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| Overall design |
(1) Analysis of tag counts from RNA or DNA input control from the MPRA experiment in C2C12s. (2) Comparison between wildtype and mutant organ transcriptomes in the Periltm1.1Vlcg strain.
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| |
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| Contributor(s) |
Groff AF, Rinn JL |
| Citation(s) |
30537984 |
| |
| Submission date |
Sep 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Abbie Groff |
| E-mail(s) |
agroff@fas.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology;
|
| Lab |
John Rinn
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (3) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19604 |
Illumina HiSeq 2500 (synthetic construct) |
|
| Samples (30)
|
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| Relations |
| BioProject |
PRJNA412390 |
| SRA |
SRP118998 |
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