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Series GSE10453 Query DataSets for GSE10453
Status Public on Feb 12, 2008
Title ChIP-chip from HCT116 cells with UbH2B and H3K4me2 coupled to expression analysis
Organism Homo sapiens
Experiment type Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
Summary Histone H2B monoubiquitylation has been implicated in transcription. We generated anti-UbH2B antibodies and applied these antibodies in ChIP-chip experiments revealing preferential association of ubiquitylated H2B with the transcribed regions of highly expressed genes.
Unlike dimethylated H3K4, UbH2B was not associated with distal promoter regions.
Keywords: Chip-chip, Expression analysis
 
Overall design ChIP from HCT116 cells using anti-UbH2B and anti-H3K4me2 (Control antibody used as well), and expression analysis.
 
Contributor(s) Minsky N, Shema E, Field Y, Schuster M, Segal E, Oren M
Citation(s)
  • Minsky N, Shema E, Field Y, Schuster M et al. Monoubiquitinated H2B is associated with the transcribed region of highly expressed genes in human cells. Nat Cell Biol 2008 Apr;10(4):483-8. PMID: 18344985
Submission date Feb 08, 2008
Last update date Mar 25, 2019
Contact name Neri Minsky
E-mail(s) nminsky@rockefeller.edu
Organization name Rockefeller University
Lab Laboratory of Biochemistry and Molecular Biology
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (2)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
GPL5082 [Hs_PromPR] Affymetrix Human Promoter 1.0R Array
Samples (7)
GSM264618 UbH2B normalized to H3
GSM264619 UbH2B normalized to Input DNA
GSM264620 UbH2B normalized to non-specific antibody
Relations
BioProject PRJNA108007

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10453_RAW.tar 929.0 Mb (http)(custom) TAR (of CEL, TXT)
Processed data provided as supplementary file
Processed data included within Sample table

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