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Series GSE104653 Query DataSets for GSE104653
Status Public on Oct 31, 2017
Title GPR56/ADGRG1 inhibits Mesenchymal Differentiation and Radioresistance in Glioblastoma [GIC7]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A mesenchymal transition occurs both during natural evolution of glioblastoma (GBM) and in response to therapy. However, the molecular mechanisms underlying mesenchymal differentiation are not well understood. We have found that the adhesion G protein-coupled receptor GPR56/ADGRG1 inhibits mesenchymal differentiation and radioresistance in glioblastoma stem-like initiating cells (GICs). Here, we have performed microarray analysis of parental- versus GPR56 knockout-GICs to identify gene expression changes upon GPR56 knockout
 
Overall design To interrogate the effect of GPR56 loss on the transcriptome of glioma initiating cells (GICs) we compared the gene expression profile of parental- and GPR56 knockout-GICs by genome-wide microarray analysis. Patient-derived GICs (parental and KO5) were cultured under adherent conditions on laminin in serum-free neural stem cell medium (in the presence of bFGF and EGF) at 37°C, 5% CO2 and 5% oxygen. Total RNA was extracted, labeled and hybridized in a SurePrint G3 Human GE 8x60k array (Agilent Technologies). Four paired biological replicates of each parental- and GPR56 knockout-GICs were used
 
Contributor(s) de la Iglesia N
Citation(s) 29166609
Submission date Oct 05, 2017
Last update date Jul 25, 2021
Contact name Nuria de la Iglesia
E-mail(s) niglesia@clinic.ub.es
Organization name IDIBAPS
Street address Rossello 153
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platforms (1)
GPL20844 Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Feature Number Version]
Samples (8)
GSM2805095 GIC7, parental-control, rep 1
GSM2805096 GIC7, GPR56 knockout, rep 1
GSM2805097 GIC7, parental-control, rep 2
Relations
BioProject PRJNA413406

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104653_RAW.tar 110.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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