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Series GSE105160 Query DataSets for GSE105160
Status Public on Oct 20, 2019
Title RNASeq of mouse, human, and non-human primate primary dermal fibroblasts to poly(I:C) transfection
Organisms Saimiri sciureus; Macaca mulatta; Macaca nemestrina; Papio anubis; Gorilla gorilla; Pan paniscus; Pan troglodytes; Pongo abelii; Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here, we performed RNA-Seq on mRNA isolated from primary dermal fibroblasts of human, mice and multiple non-human primate species transfected with polyinosinic-polycytidylic acid (polyI:C), a synthetic dsRNA analog that invokes a strong interferon-mediated response, or mock transfected.
Overall design For each of the ten species, primary fibroblasts were acquired from three donors. Cells from each donor were transfected in triplicate with 0.5 ug poly(I:C)/well in a six well plate (~0.05 ug/cm2) or mock-transfected (all transfection mixture components minus poly(I:C)) in triplicate. Thus, there were a total of 180 samples (10 species x 3 donors x 6 samples per donor).
Contributor(s) Gaska JM, Parsons L, Balev M, Cirincione A, Wang W, Schwartz RE, Ploss A
Citation(s) 31649152
Submission date Oct 19, 2017
Last update date Jul 25, 2021
Contact name Alexander Ploss
Phone 609-258-7128
Organization name Princeton University
Department Molecular Biology
Lab 110 Lewis Thomas Laboratory
Street address Washington Road
City Princeton
State/province NJ
ZIP/Postal code 08544
Country USA
Platforms (14)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19129 Illumina HiSeq 2500 (Macaca mulatta)
Samples (197)
GSM2823520 Orangutan donor PR01109 treated A
GSM2823521 Orangutan donor PR01109 treated B
GSM2823522 Orangutan donor PR01109 treated C
BioProject PRJNA414978
SRA SRP120495

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Supplementary file Size Download File type/resource
GSE105160_RAW.tar 60.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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