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| Status |
Public on Jul 19, 2018 |
| Title |
Gene and microRNA expression data from neuroendocrine transdifferentiated and untreated prostate cancer cell line LNCaP |
| Platform organisms |
Homo sapiens; synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
Prostate tumors contain foci of neuroendocrine transdifferentiation (NETD), resulting in an increase of androgen-independent neuroendocrine-like (NE) tumor cells, whose number significantly correlates with tumor aggressiveness and a lower survival rate. The mechanisms leading to NETD and the exact role of NE-like tumor cells in disease progression are not fully understood yet.
We used microarrays to analyze mRNA and microRNA expression during NE-transdifferentiation and we identified wide changes in both expression profiles including several genes and microRNAs with potential roles in NETD of prostate carcinoma.
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| Overall design |
LNCaP prostate cancer cells were differentiated to NE-like cancer cells by androgen deprivation or cultivated in control medium for RNA extraction and hybridization on Affymetrix microarrays. Succesful NE differentiation was confirmed by qRT-PCR analysis of three marker genes (PSA, AR, NSE). Total RNA from three independent NETDs and control cells was pooled for gene and miRNA expression analysis.
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| Contributor(s) |
Jaroslaw D, Klein-Hitpass L |
| Citation(s) |
30001402 |
| |
| Submission date |
Oct 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
|
| Phone |
+49 201 723 85552
|
| Organization name |
Institut fuer Zellbiologie
|
| Department |
Universitaetsklinikum
|
| Lab |
BioChip Lab
|
| Street address |
Virchowstr. 173
|
| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
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| Platforms (2) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
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| Samples (4)
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| GSM2825802 |
LNCaP cells cultivated in charcoal-stripped FCS (mRNA) |
|
| Relations |
| BioProject |
PRJNA415137 |
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