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Status |
Public on Dec 12, 2017 |
Title |
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Many patients with type 1 diabetes (T1D) have residual beta cells producing small amounts of C-peptide long after disease onset, but develop an inadequate glucagon response to hypoglycemia following T1D diagnosis. The features of these residual beta cells and alpha cells persisting in the islet endocrine compartment are largely unknown due to difficulty of comprehensive investigation. By studying the T1D pancreas and isolated islets, we show that remnant beta cells appeared to maintain several aspects of regulated insulin secretion. However, the function of T1D alpha cells was markedly reduced and these cells had alterations in transcription factors constituting and alpha and beta cell identity. In the native pancreas and after placing the T1D islets into a non-autoimmune, normoglycemic in vivo environment, there was no evidence of alpha-to-beta cell conversion. These results suggest a new explanation for the disordered T1D counterregulatory glucagon response to hypoglycemia.
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Overall design |
Total of 8 samples were analyzed, 5 were non-diabetic control donors and 3 were T1D donors. α cells were FACS-sorted and RNA was extracted from each of these samples. RNAseq was performed on all 8 samples
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Contributor(s) |
Powers AC, Brissova M, Haliyur R, Saunders D, Shrestha S |
Citation(s) |
- Brissova M, Haliyur R, Saunders D, Shrestha S et al. α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. Cell Rep 2018 Mar 6;22(10):2667-2676. PMID: 29514095
- Haliyur R, Tong X, Sanyoura M, Shrestha S et al. Human islets expressing HNF1A variant have defective β cell transcriptional regulatory networks. J Clin Invest 2019 Jan 2;129(1):246-251. PMID: 30507613
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Submission date |
Oct 25, 2017 |
Last update date |
Oct 28, 2020 |
Contact name |
Nripesh Prasad |
E-mail(s) |
nprasad@hudsonalpha.org
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Phone |
2562261750
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Organization name |
Hudson Alpha Institute for Biotechnology
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Street address |
601 Genome way
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City |
Huntsville |
State/province |
Alabama |
ZIP/Postal code |
35806 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (8)
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Relations |
BioProject |
PRJNA415746 |
SRA |
SRP121445 |